Effect of hypoxia on endothelin-1 production by pulmonary vascular endothelial cells

Jennifer L. Wiebke, Chahrzad Montrose-Rafizadeh, Pamela L. Zeitlin, William B Guggino

Research output: Contribution to journalArticle

Abstract

Endothelin-1 (ET-1), a peptide product of endothelial cells, is mitogenic for fibroblasts and smooth muscle cells. In this study we examined the effect of hypoxia on ET-1 production by bovine pulmonary vascular endothelial cells. Bovine pulmonary artery (BPAE) and microvascular endothelial (BMVE) cells were isolated, grown in tissue culture, and characterized by the presence of Factor VIII related antigen and LDL uptake. Baseline production of ET-1 by BPAE cells (measured by8 radioimunoassay) increased over time. BMVE cells produced one tenth the amount of ET-1 as produced by the pulmonary artery endothelial cells under the same conditions. In both cell types, hypoxia (0% O2) significantly reduced the amount of ET-1 at 48 h. Restoration of normoxia in 21% O2 for 48 h resulted in a return of ET-1 levels to baseline. Northern blot analysis showed decreased ET-1 mRNA in cells exposed to hypoxia for 48 h. These data demonstrate that pulmonary vascular endothelial cells respond to hypoxia by reversibly decreasing ET-1 production, and this attenuation is likely regulated at the level of transcription.

Original languageEnglish (US)
Pages (from-to)105-111
Number of pages7
JournalBiochimica et Biophysica Acta - Molecular Cell Research
Volume1134
Issue number2
DOIs
StatePublished - Mar 16 1992

Fingerprint

Endothelin-1
Endothelial Cells
Lung
Pulmonary Artery
Cell Hypoxia
Hypoxia
von Willebrand Factor
Northern Blotting
Smooth Muscle Myocytes
Fibroblasts
Messenger RNA
Peptides

Keywords

  • Endothelin-1
  • Hypoxia

ASJC Scopus subject areas

  • Biophysics
  • Cell Biology
  • Molecular Biology

Cite this

Effect of hypoxia on endothelin-1 production by pulmonary vascular endothelial cells. / Wiebke, Jennifer L.; Montrose-Rafizadeh, Chahrzad; Zeitlin, Pamela L.; Guggino, William B.

In: Biochimica et Biophysica Acta - Molecular Cell Research, Vol. 1134, No. 2, 16.03.1992, p. 105-111.

Research output: Contribution to journalArticle

Wiebke, Jennifer L. ; Montrose-Rafizadeh, Chahrzad ; Zeitlin, Pamela L. ; Guggino, William B. / Effect of hypoxia on endothelin-1 production by pulmonary vascular endothelial cells. In: Biochimica et Biophysica Acta - Molecular Cell Research. 1992 ; Vol. 1134, No. 2. pp. 105-111.
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