Effect of hydroxyurea on procyclic Trypanosoma brucei: An unconventional mechanism for achieving synchronous growth

Arnab Roy Chowdhury; Zhixing Zhao; Paul T. Englund

doi:10.1128/EC.00369-07

Effect of hydroxyurea on procyclic Trypanosoma brucei: An unconventional mechanism for achieving synchronous growth

Arnab Roy Chowdhury, Zhixing Zhao, Paul T. Englund

Research output: Contribution to journal › Article › peer-review

31 Scopus citations

Abstract

Procyclic Trypanosoma brucei cells were synchronized with 0.2 mM hydroxyurea. The cells did not arrest at the G₁/S boundary but proceeded through one round of replication and arrested near the end of S phase. The mitochondrial genome (kinetoplast DNA network) replicated, forming two progeny networks, but the repair of minicircle gaps was inhibited.

Original language	English (US)
Pages (from-to)	425-428
Number of pages	4
Journal	Eukaryotic Cell
Volume	7
Issue number	2
DOIs	https://doi.org/10.1128/EC.00369-07
State	Published - Feb 2008
Externally published	Yes

ASJC Scopus subject areas

Microbiology
Molecular Biology

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abstract = "Procyclic Trypanosoma brucei cells were synchronized with 0.2 mM hydroxyurea. The cells did not arrest at the G1/S boundary but proceeded through one round of replication and arrested near the end of S phase. The mitochondrial genome (kinetoplast DNA network) replicated, forming two progeny networks, but the repair of minicircle gaps was inhibited.",

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Effect of hydroxyurea on procyclic Trypanosoma brucei: An unconventional mechanism for achieving synchronous growth

Abstract

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