Effect of host cell lipid metabolism on alphavirus replication, virion morphogenesis, and infectivity

Ching G. Ng, Isabelle Coppens, Dhanasekaran Govindarajan, John Pisciotta, Vladimir Shulaev, Diane Griffin

Research output: Contribution to journalArticle

Abstract

The alphavirus Sindbis virus (SINV) causes encephalomyelitis in mice. Lipid-containing membranes, particularly cholesterol and sphingomyelin (SM), play important roles in virus entry, RNA replication, glycoprotein transport, and budding. Levels of SM are regulated by sphingomyelinases (SMases). Acid SMase (ASMase) deficiency results in the lipid storage disease type A Niemann-Pick disease (NPD-A), mimicked in mice by interruption of the ASMase gene. We previously demonstrated that ASMase-deficient mice are more susceptible to fatal SINV encephalomyelitis, with increased viral replication, spread, and neuronal death. To determine the mechanisms by which ASMase deficiency enhances SINV replication,wecompared NPD-A fibroblasts (NPAF) to normal human fibroblasts (NHF). NPAF accumulated cholesterol-and sphingolipid-rich late endosomes/lysosomes in the perinuclear region. SINV replication was faster and reached higher titer in NPAF than in NHF, and NPAF died more quickly. SINV RNA and protein synthesis was greater in NHF than in NPAF, but virions budding from NPAF were 26 times more infectious and were regular dense particles whereas virions from NHF were larger particles containing substantial amounts of CD63. Cellular regulation of alphavirus morphogenesis is a previously unrecognized mechanism for control of virus replication and spread.

Original languageEnglish (US)
Pages (from-to)16326-16331
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume105
Issue number42
DOIs
StatePublished - Oct 21 2008

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Alphavirus
Morphogenesis
Lipid Metabolism
Virion
Fibroblasts
Sindbis Virus
Virus Replication
Encephalomyelitis
Acids
Sphingomyelins
Type A Niemann-Pick Disease
Niemann-Pick Diseases
Cholesterol
RNA
Sphingomyelin Phosphodiesterase
Virus Internalization
Sphingolipids
Endosomes
Membrane Lipids
Lysosomes

Keywords

  • Acidic sphingomyelinase deficiency
  • CD63
  • Cholesterol
  • Sindbis virus replication
  • Sphingomyelin

ASJC Scopus subject areas

  • General

Cite this

Effect of host cell lipid metabolism on alphavirus replication, virion morphogenesis, and infectivity. / Ng, Ching G.; Coppens, Isabelle; Govindarajan, Dhanasekaran; Pisciotta, John; Shulaev, Vladimir; Griffin, Diane.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 105, No. 42, 21.10.2008, p. 16326-16331.

Research output: Contribution to journalArticle

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