@article{836f0f920e8a4544a653e2a04f907295,
title = "Effect of ethanol administration on the activity of hepatic lysosomal enzymes",
abstract = "The activities of hepatic lysosomal enzymes were determined in rats after the acute administration of ethanol and its feeding for periods of 1 and 6 months. The acute administration of ethanol and its feeding for 1 month did not result in any changes in lysosomal enzymes. By contrast the feeding of ethanol for a period of 6 months resulted in increases in the total activities of β-glucuronidase, β-N-acetylglucosaminidase and arylsulfatase A measured in the homogenates, and in the specific activities of these enzymes plus acid phosphatase measured in the lysosomal fraction. In addition, there were increases in the free activities of β-glucuronidase and acid phosphatase measured in the homogenates. Hyaluronidase activity was not changed by ethanol feeding. The increases in lysosomal enzymes were accompanied by histologic changes consisting only of mild fatty infiltration.",
author = "Esteban Mezey and Potter, {James J.} and Ammon, {Randall A.}",
note = "Funding Information: Animals and diets The animals were sacrificed by a blow on the head Male albino rats weighing between 90 and 1\]0g after 14hr of fasting. The livers were immediately initially were kept in individual wire cages at a con-removed, rinsed in cold 0.25 M sucrose and weighed. stant temperature. The following acute and chronic A small piece was fixed in 10% formaldehyde for his-experiments were carried out. tological examination and the remainder homo-Acute experiment. Sixteen rats were fed Purina genized in a Potter-Elvehjem homogenizer in a Chow ad lib. Ethanol (4 g/kg of body weight) was volume of 0.25 M sucrose solution containing administered twice, 8 hr apart, by stomach tube as 1 × 103 M EDTA, pH 7.0, equivalent to four times a 40% solution to one-half the rats. The control rats the liver weight. Five ml of the homogenate were saved were given isocaloric amounts of sucrose. The animals for the determination of total and free lysosomal were sacrificed 24 hr after the first dose. enzyme activities, and the remainder subjected to dif-Chronic experiments. Thirty-two rats ferential centrifugation at 4 °, using a Sorvall refriger-ated centrifuge, according to Sawant et al. \[i1\] to * This work was supported by grant AA 00 626 from obtain the lysosomal-rich light mitochondrial fraction the United States Public Health Service and a supplemen-10 min, and the resulting supernatant was centrifuged (FI). The homogenate was centrifuged at 750 0 for tal research grant from the National Council on Alcoholism. at 3,300 9 for 10 min, and again at 16,300 9 for 20 rain. ~{"} Recipient of a research fellowship from the The resulting precipitate was resuspended in 5 ml of Council on Alcoholism, Baltimore, MD. 0.15N NaC1. Three ml were separated, sonicated :~ Same as the composition used in the high alcohol (three times for 30 see), and used for the determina-liquid diet of General Biochemicals, Chagrin Falls, Ohio. tion of lysosomal enzymes. The remaining 2 ml were 2663 Copyright: Copyright 2014 Elsevier B.V., All rights reserved.",
year = "1976",
month = dec,
day = "1",
doi = "10.1016/0006-2952(76)90524-4",
language = "English (US)",
volume = "25",
pages = "2663--2667",
journal = "Biochemical Pharmacology",
issn = "0006-2952",
publisher = "Elsevier Inc.",
number = "23",
}