Effect of dl-buthionine-S,R-sulfoximine on 1,2-dimethyl-hydrazine-induced colonic tumors in rats

M. F. Chen; L. T. Chen; H. S. Ying; H. W. Boyce

doi:10.1016/0304-3835(90)90165-T

Effect of dl-buthionine-S,R-sulfoximine on 1,2-dimethyl-hydrazine-induced colonic tumors in rats

M. F. Chen, L. T. Chen, H. S. Ying, H. W. Boyce

School of Medicine

Research output: Contribution to journal › Article › peer-review

1 Scopus citations

Abstract

The effect of dl-buthionine-S,R-sulfoximine (BSO), a specific inhibitor of glutathione biosynthesis, on colon tumor development was studied in rats. Weanling male Sprague-Dawley rats were randomly assigned to one of three groups following a week of adaptation. Group 1 rats received BSO (4.5 mM) daily in the drinking water one week before 1,2-dimethylhydrazine (DMH) injections and continued to receive BSO daily until sacrificed; group 2 rats received BSO (4.5 mM) after the last DMH injection, and continued to receive BSO daily until sacrificed; group 3 rats did not receive BSO. All experimental rats received 20 weekly subcutaneous injections of DMH (20 mg/kg body wt.) for colon tumor induction. The tumor incidence was lower in group 1 (54%) than in group 2 (97%) or group 3 (96%). The median tumor size is significantly smaller in group 1 (11 mm²) than group 3 (46 mm²). The group 2 had the largest median tumor size (65 mm²).

Original language	English (US)
Pages (from-to)	237-242
Number of pages	6
Journal	Cancer Letters
Volume	49
Issue number	3
DOIs	https://doi.org/10.1016/0304-3835(90)90165-T
State	Published - Mar 1990

Keywords

1,2-dimethylhydrazine
buthionine sulfoximine
colon carcinoma
glutathione
rats

ASJC Scopus subject areas

Oncology
Cancer Research

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10.1016/0304-3835(90)90165-T

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title = "Effect of dl-buthionine-S,R-sulfoximine on 1,2-dimethyl-hydrazine-induced colonic tumors in rats",

abstract = "The effect of dl-buthionine-S,R-sulfoximine (BSO), a specific inhibitor of glutathione biosynthesis, on colon tumor development was studied in rats. Weanling male Sprague-Dawley rats were randomly assigned to one of three groups following a week of adaptation. Group 1 rats received BSO (4.5 mM) daily in the drinking water one week before 1,2-dimethylhydrazine (DMH) injections and continued to receive BSO daily until sacrificed; group 2 rats received BSO (4.5 mM) after the last DMH injection, and continued to receive BSO daily until sacrificed; group 3 rats did not receive BSO. All experimental rats received 20 weekly subcutaneous injections of DMH (20 mg/kg body wt.) for colon tumor induction. The tumor incidence was lower in group 1 (54%) than in group 2 (97%) or group 3 (96%). The median tumor size is significantly smaller in group 1 (11 mm2) than group 3 (46 mm2). The group 2 had the largest median tumor size (65 mm2).",

keywords = "1,2-dimethylhydrazine, buthionine sulfoximine, colon carcinoma, glutathione, rats",

author = "Chen, {M. F.} and Chen, {L. T.} and Ying, {H. S.} and Boyce, {H. W.}",

note = "Funding Information: This project was supported in part by the American Cancer Society, Florida Division, Inc. The authors thank Gina Lund for her secretarial assistance.",

year = "1990",

month = mar,

doi = "10.1016/0304-3835(90)90165-T",

language = "English (US)",

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pages = "237--242",

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AU - Chen, L. T.

AU - Ying, H. S.

AU - Boyce, H. W.

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PY - 1990/3

Y1 - 1990/3

N2 - The effect of dl-buthionine-S,R-sulfoximine (BSO), a specific inhibitor of glutathione biosynthesis, on colon tumor development was studied in rats. Weanling male Sprague-Dawley rats were randomly assigned to one of three groups following a week of adaptation. Group 1 rats received BSO (4.5 mM) daily in the drinking water one week before 1,2-dimethylhydrazine (DMH) injections and continued to receive BSO daily until sacrificed; group 2 rats received BSO (4.5 mM) after the last DMH injection, and continued to receive BSO daily until sacrificed; group 3 rats did not receive BSO. All experimental rats received 20 weekly subcutaneous injections of DMH (20 mg/kg body wt.) for colon tumor induction. The tumor incidence was lower in group 1 (54%) than in group 2 (97%) or group 3 (96%). The median tumor size is significantly smaller in group 1 (11 mm2) than group 3 (46 mm2). The group 2 had the largest median tumor size (65 mm2).

AB - The effect of dl-buthionine-S,R-sulfoximine (BSO), a specific inhibitor of glutathione biosynthesis, on colon tumor development was studied in rats. Weanling male Sprague-Dawley rats were randomly assigned to one of three groups following a week of adaptation. Group 1 rats received BSO (4.5 mM) daily in the drinking water one week before 1,2-dimethylhydrazine (DMH) injections and continued to receive BSO daily until sacrificed; group 2 rats received BSO (4.5 mM) after the last DMH injection, and continued to receive BSO daily until sacrificed; group 3 rats did not receive BSO. All experimental rats received 20 weekly subcutaneous injections of DMH (20 mg/kg body wt.) for colon tumor induction. The tumor incidence was lower in group 1 (54%) than in group 2 (97%) or group 3 (96%). The median tumor size is significantly smaller in group 1 (11 mm2) than group 3 (46 mm2). The group 2 had the largest median tumor size (65 mm2).

KW - 1,2-dimethylhydrazine

KW - buthionine sulfoximine

KW - colon carcinoma

KW - glutathione

KW - rats

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Effect of dl-buthionine-S,R-sulfoximine on 1,2-dimethyl-hydrazine-induced colonic tumors in rats

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Keywords

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