Effect of cyclosporin and interleukin-2 on the restoration of in vitro immune responses to cytomegalovirus

P. J. Converse, A. D. Hess

Research output: Contribution to journalArticle

Abstract

Previous studies have shown that cyclosporin (CsA) inhibits lymphoproliferation to cytomegalovirus (CMV)-infected, glutaraldehyde-fixed, and irradiated fibroblasts (CMVFF(x)) in vitro. Generation of cytotoxic cell activity is impaired in cultures with CsA, but the induction of suppressor cells is not. In the present studies we tested the ability of interleukin-2 (IL-2) and supernatants of lymphocytes stimulated by CMVFF(x) with or without CsA (1 μg/ml) to restore functional activities of lymphocytes from primary cultures treated or not treated with CsA. IL-2 significantly enhanced lymphoproliferation, cell-mediated cytotoxicity to CMV-infected fibroblasts (CMVF), natural killer cell activity, and the activity of cells capable of suppressing the response of fresh autologous cells to CMVFF(x) of cells derived from control and CsA-treated primary cultures. IL-2 was found in day-2 supernatants of control cultures but not CsA-treated cultures. Day-2 control supernatants were capable of significantly enhancing proliferation and suppressor cell activity but were less efficient at restoring cytotoxic cell function. Day-2 supernatants from CsA-treated cultures were not able to enhance lymphoproliferation or cytotoxic cell function but did induce significant levels of suppressor cell activity. The results indicate the presence of different functional mediators in the culture supernatants. The ability of IL-2 to restore lymphocyte effector functions against a clinically important virus may have important therapeutic implications in the treatment of this viral infection in immunodeficiency diseases and in restoration of immune competence after transplantation.

Original languageEnglish (US)
Pages (from-to)109-118
Number of pages10
JournalScandinavian Journal of Immunology
Volume21
Issue number2
StatePublished - 1985

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Cytomegalovirus
Cyclosporine
Interleukin-2
Aptitude
Lymphocytes
Fibroblasts
In Vitro Techniques
Glutaral
Virus Diseases
Natural Killer Cells
Mental Competency
Transplantation
Cell Proliferation
Viruses

ASJC Scopus subject areas

  • Immunology

Cite this

Effect of cyclosporin and interleukin-2 on the restoration of in vitro immune responses to cytomegalovirus. / Converse, P. J.; Hess, A. D.

In: Scandinavian Journal of Immunology, Vol. 21, No. 2, 1985, p. 109-118.

Research output: Contribution to journalArticle

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