Effect of cyclooxygenase inhibition on brain cell membrane lipid peroxidation during hypoxia in newborn piglets

Jane E. McGowan, Joseph C. McGowan, Om P. Mishra, M. Delivoria-Papadopoulos

Research output: Contribution to journalArticle

Abstract

To test the hypothesis that indomethacin, an inhibitor of cyclooxygenase, reduces free radical-induced brain cell membrane changes during cerebral hypoxia, we determined levels of brain cell membrane lipid peroxidation products and Na+,K+-ATPase activity as indicators of free radical production and membrane function, respectively, in 29 newborn piglets divided into 4 groups. Eight saline-and 4 indomethacin-treated normoxic animals served as controls; 8 saline-pretreated piglets and 9 piglets pretreated with indomethacin were exposed to hypoxic hypoxia for 60 min. Cerebral hypoxia was documented using31P-NMR spectroscopy. In saline-pre-treated hypoxic animals Na+,K+-ATPase activity decreased significantly and levels of membrane lipid peroxidation products increased significantly compared to normoxic controls. Indomethacin pretreatment prevented the hypoxia-induced increase in membrane lipid peroxidation products but had no effect on the decrease in Na+,K+-ATPase activity. Thus the apparent reduction in free radical production by indomethacin pretreatment did not prevent the hypoxia-induced change in Na+,K+-ATPase activity.

Original languageEnglish (US)
Pages (from-to)367-375
Number of pages9
JournalNeonatology
Volume66
Issue number6
DOIs
StatePublished - 1994
Externally publishedYes

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Keywords

  • Brain
  • Free radicals
  • Hypoxia
  • Indomethacin
  • K<sup>+</sup>-ATPase
  • Na<sup>+</sup>
  • Newborn
  • NMR spectroscopy

ASJC Scopus subject areas

  • Developmental Biology
  • Pediatrics, Perinatology, and Child Health

Cite this

McGowan, J. E., McGowan, J. C., Mishra, O. P., & Delivoria-Papadopoulos, M. (1994). Effect of cyclooxygenase inhibition on brain cell membrane lipid peroxidation during hypoxia in newborn piglets. Neonatology, 66(6), 367-375. https://doi.org/10.1159/000244130