Effect of cholera toxin on histamine release from bone marrow-derived mouse mast cells

H. Saito, F. Okajima, T. F.P. Molski, R. I. Sha'afi, M. Ui, T. Ishizaka

Research output: Contribution to journalArticle

Abstract

Bone marrow-derived mouse mast cells were sensitized with monoclonal mouse IgE antibody and treated with cholera toxin (CT), which ADP-ribosylated the α-subunit of the stimulatory guanine nucleotide-binding regulatory protein G(s), prior to challenge with either antigen or thrombin. The CT treatment inceased intracellular cAMP levels, but neither enhanced nor inhibited antigen-induced histamine release or arachidonate release. The same treatment of the sensitized bone marrow-derived mouse mast cells with CT markedly enhanced thrombin-induced histamine release without affecting arachidonate release. The CT treatment failed to affect antigen-induced and thrombin-induced generation of inositol triphosphate and of diacylglycerol or mobilization of intracellular Ca2+. The results indicate that G(s) in bone marrow-derived mouse mast cells is not involved involved in the transduction of the antigen-induced or thrombin-induced triggering signal to phospholipase C, which initiates the enhancement of phosphatidylinositol turnover. The enhancement of thrombin-induced histamine release by CT treatment with the observations that thrombin-induced histamine release was inhibited by pretreatment of the cells with pertussis toxin suggest that the involvement of a guanine nucleotide-binding regulatory protein in thrombin-induced biochemical events is an event distal to Ca2+ mobilization.

Original languageEnglish (US)
Pages (from-to)2504-2508
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume85
Issue number8
DOIs
StatePublished - Jan 1 1988

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