Effect of CD40 ligand and other immunomodulators on Pneumocystis carinii infection in rat model

Helieh S. Oz, Walter T. Hughes, Jerold E. Rehg, Elaine K. Thomas

Research output: Contribution to journalArticlepeer-review

Abstract

The corticosteroid-treated animal is well established as an experimental model for the study of Pneumocystis carinii pneumonitis (PCP). Latent or acquired infection with P. carinii in the murine lung progresses to fatal pneumonitis when the host is profoundly immunocompromized. In this study the effects of five immunomodulators; recombinant CD40 ligand (CD40L), bryostatin 1, recombinant FLT3 ligand (FLT3L), recombinant granulocyte colony-stimulating factor (G-CSF) and recombinant interleukin-15 (IL-15) were investigated against PCP in a dexamethasone immuno-suppressed Sprague-Dawley rat model. The majority of rats (70%) treated with CD40L at the onset of dexamethasone immunosuppression were protected against PCP. When CD40L was given after 10 days of immunosuppression, only 40% of the rats resolved the infection. However, 95% of the control animals developed PCP. Immunosuppressed rats treated with bryostatin 1, an immune activator had a partial (50%) protection against P. carinii infection. In contrast, daily administration of FLT3L, IL-15 or G-CSF provided no protection against P. carinii infection. (C) 2000 Academic Press.

Original languageEnglish (US)
Pages (from-to)187-190
Number of pages4
JournalMicrobial Pathogenesis
Volume29
Issue number3
DOIs
StatePublished - 2000

Keywords

  • Bryostatin 1
  • CD40L
  • FLT3L
  • IL-15
  • P. carinii
  • PCP

ASJC Scopus subject areas

  • Microbiology
  • Infectious Diseases

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