Effect of catechins and high-temperature-processed green tea extract on scavenging reactive oxygen species and preventing Aβ1–42 fibrils’ formation in brain microvascular endothelium

Seon Bong Lee, Eun Hye Choi, Kang Hyun Jeong, Kwang Sik Kim, Soon Mi Shim, Gun Hee Kim

Research output: Contribution to journalArticle

Abstract

The present study investigated the effect of high-temperature-processed green tea extract (HTP_GTE) and its bioactive components on the reduction of reactive oxygen species (ROS) and amyloid-beta (Aβ) protein in human microvascular endothelial cells. Compared to Aβ1–42-only treatment, pretreatment of HTP_GTE was revealed to effectively inhibit ROS generation (P<0.05). HTP_GTE and catechins not only inhibit Aβ1–42 fibril formation but also destabilize preformed Aβ1–42 fibrils. The presence of HTP_GTE, Aβ1–42 fibril formation was significantly inhibited in a dose-dependent manner at 12.5–100 μg/ml of HTP_GTE, showing 86–56%, respectively. Treatment of various concentrations of HTP_GTE and catechins steadily destabilized the preformed Aβ1–42 fibrils for 24 h in a dose-dependent manner. It was observed that the gallated groups such as epigallocatechin gallate, epicatechin gallate, gallocatechin gallate, and catechin gallate more effectively disturbed Aβ1–42 fibril formation and destabilized the preformed Aβ1–42 fibrils than the non-gallated group. Taken together, these findings supported that sterilized green tea could be promising natural anti-amyloidogenic agents associated with therapeutic approaches in Alzheimer’s disease by scavenging ROS generation and Aβ fibril in the brain tissue.

Original languageEnglish (US)
JournalNutritional Neuroscience
DOIs
StateAccepted/In press - Jan 1 2018

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Catechin
Tea
Endothelium
Reactive Oxygen Species
Temperature
Brain
Serum Amyloid A Protein
Amyloid beta-Peptides
Alzheimer Disease
Endothelial Cells

Keywords

  • Aβ fibrils
  • Blood-brain barrier
  • Catechins
  • High-temperature-processed green tea extract
  • Reactive oxygen species

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Neuroscience(all)
  • Nutrition and Dietetics

Cite this

@article{6223625e358248b4a5a3dfa05b53ad7e,
title = "Effect of catechins and high-temperature-processed green tea extract on scavenging reactive oxygen species and preventing Aβ1–42 fibrils’ formation in brain microvascular endothelium",
abstract = "The present study investigated the effect of high-temperature-processed green tea extract (HTP_GTE) and its bioactive components on the reduction of reactive oxygen species (ROS) and amyloid-beta (Aβ) protein in human microvascular endothelial cells. Compared to Aβ1–42-only treatment, pretreatment of HTP_GTE was revealed to effectively inhibit ROS generation (P<0.05). HTP_GTE and catechins not only inhibit Aβ1–42 fibril formation but also destabilize preformed Aβ1–42 fibrils. The presence of HTP_GTE, Aβ1–42 fibril formation was significantly inhibited in a dose-dependent manner at 12.5–100 μg/ml of HTP_GTE, showing 86–56{\%}, respectively. Treatment of various concentrations of HTP_GTE and catechins steadily destabilized the preformed Aβ1–42 fibrils for 24 h in a dose-dependent manner. It was observed that the gallated groups such as epigallocatechin gallate, epicatechin gallate, gallocatechin gallate, and catechin gallate more effectively disturbed Aβ1–42 fibril formation and destabilized the preformed Aβ1–42 fibrils than the non-gallated group. Taken together, these findings supported that sterilized green tea could be promising natural anti-amyloidogenic agents associated with therapeutic approaches in Alzheimer’s disease by scavenging ROS generation and Aβ fibril in the brain tissue.",
keywords = "Aβ fibrils, Blood-brain barrier, Catechins, High-temperature-processed green tea extract, Reactive oxygen species",
author = "Lee, {Seon Bong} and Choi, {Eun Hye} and Jeong, {Kang Hyun} and Kim, {Kwang Sik} and Shim, {Soon Mi} and Kim, {Gun Hee}",
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T1 - Effect of catechins and high-temperature-processed green tea extract on scavenging reactive oxygen species and preventing Aβ1–42 fibrils’ formation in brain microvascular endothelium

AU - Lee, Seon Bong

AU - Choi, Eun Hye

AU - Jeong, Kang Hyun

AU - Kim, Kwang Sik

AU - Shim, Soon Mi

AU - Kim, Gun Hee

PY - 2018/1/1

Y1 - 2018/1/1

N2 - The present study investigated the effect of high-temperature-processed green tea extract (HTP_GTE) and its bioactive components on the reduction of reactive oxygen species (ROS) and amyloid-beta (Aβ) protein in human microvascular endothelial cells. Compared to Aβ1–42-only treatment, pretreatment of HTP_GTE was revealed to effectively inhibit ROS generation (P<0.05). HTP_GTE and catechins not only inhibit Aβ1–42 fibril formation but also destabilize preformed Aβ1–42 fibrils. The presence of HTP_GTE, Aβ1–42 fibril formation was significantly inhibited in a dose-dependent manner at 12.5–100 μg/ml of HTP_GTE, showing 86–56%, respectively. Treatment of various concentrations of HTP_GTE and catechins steadily destabilized the preformed Aβ1–42 fibrils for 24 h in a dose-dependent manner. It was observed that the gallated groups such as epigallocatechin gallate, epicatechin gallate, gallocatechin gallate, and catechin gallate more effectively disturbed Aβ1–42 fibril formation and destabilized the preformed Aβ1–42 fibrils than the non-gallated group. Taken together, these findings supported that sterilized green tea could be promising natural anti-amyloidogenic agents associated with therapeutic approaches in Alzheimer’s disease by scavenging ROS generation and Aβ fibril in the brain tissue.

AB - The present study investigated the effect of high-temperature-processed green tea extract (HTP_GTE) and its bioactive components on the reduction of reactive oxygen species (ROS) and amyloid-beta (Aβ) protein in human microvascular endothelial cells. Compared to Aβ1–42-only treatment, pretreatment of HTP_GTE was revealed to effectively inhibit ROS generation (P<0.05). HTP_GTE and catechins not only inhibit Aβ1–42 fibril formation but also destabilize preformed Aβ1–42 fibrils. The presence of HTP_GTE, Aβ1–42 fibril formation was significantly inhibited in a dose-dependent manner at 12.5–100 μg/ml of HTP_GTE, showing 86–56%, respectively. Treatment of various concentrations of HTP_GTE and catechins steadily destabilized the preformed Aβ1–42 fibrils for 24 h in a dose-dependent manner. It was observed that the gallated groups such as epigallocatechin gallate, epicatechin gallate, gallocatechin gallate, and catechin gallate more effectively disturbed Aβ1–42 fibril formation and destabilized the preformed Aβ1–42 fibrils than the non-gallated group. Taken together, these findings supported that sterilized green tea could be promising natural anti-amyloidogenic agents associated with therapeutic approaches in Alzheimer’s disease by scavenging ROS generation and Aβ fibril in the brain tissue.

KW - Aβ fibrils

KW - Blood-brain barrier

KW - Catechins

KW - High-temperature-processed green tea extract

KW - Reactive oxygen species

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