Effect of calcium antagonists on RNA synthesis of NIH 3T3 cells

N. S. Andrawis, D. R. Abernethy

Research output: Contribution to journalArticlepeer-review

Abstract

Calcium antagonists have been shown to induce a decrease in peripheral vascular resistance as well as a decrease in synthesis of vascular-wall matrix proteins. It has been shown previously that calcium antagonists decrease RNA synthesis of cultured, vascular, smooth-muscle cells. Here, these findings are extended to the investigation of whether calcium antagonists produce their vascular effects through their action on vascular, smooth-muscle cells only or whether they regulate fibroblast cells as well. It is demonstrated that in a concentration-dependent manner verapamil, diltiazem, and nifedipine each induced a decrease in RNA synthesis of quiescent and serum-stimulated NIH 3T3 cells, a fibroblast cell line shown to express voltage-dependent Ca2+ channels. Verapamil and nifedipine (10-5 M) and diltiazem (10-4 M) caused a marked decrease of basal and serum- induced increase in [3H]uridine uptake of NIH 3T3 cells. This is the first report to demonstrate that calcium antagonists have a direct effect on a fibroblast cell line leading to a decrease of RNA synthesis. Such findings suggest that calcium-antagonist vascular effects extend beyond vascular smooth muscle cells to connective tissues associated with extracellular- matrix protein production.

Original languageEnglish (US)
Pages (from-to)137-140
Number of pages4
JournalAmerican Journal of the Medical Sciences
Volume306
Issue number3
DOIs
StatePublished - 1993

Keywords

  • Calcium antagonists
  • NIH 3T3
  • RNA synthesis

ASJC Scopus subject areas

  • Medicine(all)

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