Effect of bcl-2 overexpression in mice on ovotoxicity caused by 4-vinylcyclohexene

Jodi A. Flaws; Samuel L. Marion; Kimberly P. Miller; Patricia J. Christian; Janice K. Babus; Patricia B. Hoyer

doi:10.1016/j.taap.2006.03.003

Effect of bcl-2 overexpression in mice on ovotoxicity caused by 4-vinylcyclohexene

Jodi A. Flaws, Samuel L. Marion, Kimberly P. Miller, Patricia J. Christian, Janice K. Babus, Patricia B. Hoyer

Research output: Contribution to journal › Article › peer-review

10 Scopus citations

Abstract

The occupational chemical 4-vinylcyclohexene (VCH) destroys small preantral ovarian follicles in mice following repeated daily dosing. The cell survival gene bcl-2 is thought to protect against follicular death during embryogenesis because primordial follicle numbers in newborn bcl-2 overexpressing (OE) mice are greater than in wild-type (WT) controls. Thus, this study was designed to determine if overexpression of bcl-2 protects against VCH-induced follicle loss during embryonic development. Pregnant bcl-2 OE or WT mice were dosed (p.o.) daily with VCH (500 mg/kg) or sesame oil (vehicle control) on days 8-18 of pregnancy. Ovaries were collected from moms and female pups on pup postnatal day (PND) 8. Nonpregnant OE and WT females were also treated with VCH (500 mg/kg p.o.) or vehicle and evaluated in the same manner. As previously reported, ovaries from PND8 OE female pups contained 50% more primordial follicles than WT pups (P

Original language	English (US)
Pages (from-to)	51-56
Number of pages	6
Journal	Toxicology and Applied Pharmacology
Volume	215
Issue number	1
DOIs	https://doi.org/10.1016/j.taap.2006.03.003
State	Published - Aug 15 2006
Externally published	Yes

Keywords

4-vinylcyclohexene (VCH)
Female reproduction
Follicle
Mouse
Ovary

ASJC Scopus subject areas

Pharmacology
Toxicology

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10.1016/j.taap.2006.03.003

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title = "Effect of bcl-2 overexpression in mice on ovotoxicity caused by 4-vinylcyclohexene",

abstract = "The occupational chemical 4-vinylcyclohexene (VCH) destroys small preantral ovarian follicles in mice following repeated daily dosing. The cell survival gene bcl-2 is thought to protect against follicular death during embryogenesis because primordial follicle numbers in newborn bcl-2 overexpressing (OE) mice are greater than in wild-type (WT) controls. Thus, this study was designed to determine if overexpression of bcl-2 protects against VCH-induced follicle loss during embryonic development. Pregnant bcl-2 OE or WT mice were dosed (p.o.) daily with VCH (500 mg/kg) or sesame oil (vehicle control) on days 8-18 of pregnancy. Ovaries were collected from moms and female pups on pup postnatal day (PND) 8. Nonpregnant OE and WT females were also treated with VCH (500 mg/kg p.o.) or vehicle and evaluated in the same manner. As previously reported, ovaries from PND8 OE female pups contained 50% more primordial follicles than WT pups (P ",

keywords = "4-vinylcyclohexene (VCH), Female reproduction, Follicle, Mouse, Ovary",

author = "Flaws, {Jodi A.} and Marion, {Samuel L.} and Miller, {Kimberly P.} and Christian, {Patricia J.} and Babus, {Janice K.} and Hoyer, {Patricia B.}",

year = "2006",

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language = "English (US)",

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AU - Flaws, Jodi A.

AU - Marion, Samuel L.

AU - Miller, Kimberly P.

AU - Christian, Patricia J.

AU - Babus, Janice K.

AU - Hoyer, Patricia B.

PY - 2006/8/15

Y1 - 2006/8/15

N2 - The occupational chemical 4-vinylcyclohexene (VCH) destroys small preantral ovarian follicles in mice following repeated daily dosing. The cell survival gene bcl-2 is thought to protect against follicular death during embryogenesis because primordial follicle numbers in newborn bcl-2 overexpressing (OE) mice are greater than in wild-type (WT) controls. Thus, this study was designed to determine if overexpression of bcl-2 protects against VCH-induced follicle loss during embryonic development. Pregnant bcl-2 OE or WT mice were dosed (p.o.) daily with VCH (500 mg/kg) or sesame oil (vehicle control) on days 8-18 of pregnancy. Ovaries were collected from moms and female pups on pup postnatal day (PND) 8. Nonpregnant OE and WT females were also treated with VCH (500 mg/kg p.o.) or vehicle and evaluated in the same manner. As previously reported, ovaries from PND8 OE female pups contained 50% more primordial follicles than WT pups (P

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Effect of bcl-2 overexpression in mice on ovotoxicity caused by 4-vinylcyclohexene

Abstract

Keywords

ASJC Scopus subject areas

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