Effect of bcl-2 overexpression in mice on ovotoxicity caused by 4-vinylcyclohexene

Jodi A. Flaws, Samuel L. Marion, Kimberly P. Miller, Patricia J. Christian, Janice K. Babus, Patricia B. Hoyer

Research output: Contribution to journalArticle

Abstract

The occupational chemical 4-vinylcyclohexene (VCH) destroys small preantral ovarian follicles in mice following repeated daily dosing. The cell survival gene bcl-2 is thought to protect against follicular death during embryogenesis because primordial follicle numbers in newborn bcl-2 overexpressing (OE) mice are greater than in wild-type (WT) controls. Thus, this study was designed to determine if overexpression of bcl-2 protects against VCH-induced follicle loss during embryonic development. Pregnant bcl-2 OE or WT mice were dosed (p.o.) daily with VCH (500 mg/kg) or sesame oil (vehicle control) on days 8-18 of pregnancy. Ovaries were collected from moms and female pups on pup postnatal day (PND) 8. Nonpregnant OE and WT females were also treated with VCH (500 mg/kg p.o.) or vehicle and evaluated in the same manner. As previously reported, ovaries from PND8 OE female pups contained 50% more primordial follicles than WT pups (P 

Original languageEnglish (US)
Pages (from-to)51-56
Number of pages6
JournalToxicology and Applied Pharmacology
Volume215
Issue number1
DOIs
StatePublished - Aug 15 2006
Externally publishedYes

Keywords

  • 4-vinylcyclohexene (VCH)
  • Female reproduction
  • Follicle
  • Mouse
  • Ovary

ASJC Scopus subject areas

  • Pharmacology
  • Toxicology

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  • Cite this

    Flaws, J. A., Marion, S. L., Miller, K. P., Christian, P. J., Babus, J. K., & Hoyer, P. B. (2006). Effect of bcl-2 overexpression in mice on ovotoxicity caused by 4-vinylcyclohexene. Toxicology and Applied Pharmacology, 215(1), 51-56. https://doi.org/10.1016/j.taap.2006.03.003