Effect of amitriptyline on the messenger RNA of thyroid hormone-responsive genes in rat cerebral tissue

Julio Bobes, Una D. McCann

Research output: Contribution to journalReview articlepeer-review

Abstract

To determine the molecular mechanisms of the potentiating effect of thyroid hormones (TH) on the therapeutic efficacy of tricyclic antidepressants (TCA), the expression of two known TH-responsive mRNAs was measured in control rats and rats treated with triiodothyronine (T3, 10 μg/100 g for 10 days), amitriptyline (10 mg/kg for 10 days), or combined T3 and amitriptyline. Northern blot analysis was carried out to measure the cerebral tissue content of a novel translational repressor (NAT-1) and another thyroid hormone-responsive (THR) mRNA. Rats treated with the combination of T3 and amitriptyline had significantly higher NAT-1 expression (2691.1 ± 134.1 arbitrary units) than rats treated with T3 only (1688.5 ± 77.8) or with amitriptyline only (1452.5 ± 87.5) or the untreated control rats (731.3 ± 23.0), P < 0.01. Amitriptyline treatment did not alter the expression of THR mRNA or THR protein in either control or T3-treated rats. It is concluded that alterations in the expression of selective T3 responsive genes in cerebral tissue could be a mechanism of the known T3 potentiation of the therapeutic efficacy of TCA.

Original languageEnglish (US)
Pages (from-to)333-338
Number of pages6
JournalCurrent opinion in psychiatry
Volume13
Issue number3
DOIs
StatePublished - Jan 1 2000

Keywords

  • Depression
  • Gene expression
  • Thyroid hormones
  • Tricyclic antidepressants

ASJC Scopus subject areas

  • Psychiatry and Mental health

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