Effect of age and cognitive status on basal level AP-1 activity in rat hippocampus

D. R. Smith, E. C. Hoyt, M. Gallagher, R. F. Schwabe, P. K. Lund

Research output: Contribution to journalArticle

Abstract

Activator protein -1 (AP-1) was examined at multiple levels (mRNA, DNA binding, composition) in hippocampus of young and aged rats that were behaviorally characterized for spatial memory. GFAP mRNA was measured as a gene product known to increase with aging and to be regulated by AP-1. The activity of Jun-amino terminal-kinase (JNK) was also assessed. Levels of c-jun and c-fos mRNAs were unchanged with aging or spatial learning ability. Abundance of GFAP mRNA was significantly increased in aged hippocampus but did not correlate with spatial learning. Total AP-1 binding activity was unaltered with age or cognitive ability. In hippocampus of young, aged unimpaired and aged impaired rats, AP-1 consists mainly of c-Jun, phosphorylated c-Jun (p-c-Jun), JunD, and smaller amounts of c-Fos. JNK is constitutively active in young and aged hippocampus. We conclude that the basal expression of c-fos and c-jun mRNA, overall AP-1 binding activity and AP-1 composition are not influenced by aging or cognitive ability.

Original languageEnglish (US)
Pages (from-to)773-786
Number of pages14
JournalNeurobiology of Aging
Volume22
Issue number5
DOIs
StatePublished - Nov 24 2001

Keywords

  • Activator protein-1 (AP-1)
  • Aging
  • Behavior
  • Fos isoforms
  • GFAP
  • Jun isoforms
  • Long-Evans rats
  • Spatial learning
  • Water maze
  • c-fos
  • c-jun

ASJC Scopus subject areas

  • Neuroscience(all)
  • Aging
  • Clinical Neurology
  • Developmental Biology
  • Geriatrics and Gerontology

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