TY - JOUR
T1 - Effect of a Recombinant CD4-IgG on In Vitro T Helper Cell Function
T2 - Data from a Phase I/II Study of Patients with AIDS
AU - Clerici, Mario
AU - Yarchoan, Robert
AU - Blatt, Stephen
AU - Hendrix, Craig W.
AU - Ammann, Arthur J.
AU - Broder, Samuel
AU - Shearer, Gene M.
PY - 1993/10
Y1 - 1993/10
N2 - Ten patients with AIDS were enrolled in a phase I/II protocol of recombinant CD4-IgG (rCD4-IgG) treatment. Patients’ peripheral blood leukocytes (PBL) were tested before, during, and after therapy with rCD4-IgG for T helper (TH) cell function assessed by antigen- and mitogen- stimulated proliferation and interleukin-2 production in response to influenza A virus, allogeneic PBL (alloantigens), and phytohemagglutinin. Although clinical benefit was not evident, rCD4-lgG treatment was associated with rapid and potent improved TH cell function for two of three stimuli tested in 90% of the patients. These data are complemented by an in vitro experimental model that demonstrates the opposing immunologic effects ofrgp120 and rCD4-lgG on TH cell function of PBL from uninfected individuals. Thus, restoration of TH cell function by rCD4-lgG in the absence ofincreased CD4 cell counts could be due to removal ofan immunosuppressive factor, possibly gp120. These findings suggest that rCD4-lgG can induce partial restoration of immune function in AIDS patients, even in the absence of apparent short-term clinical benefit.
AB - Ten patients with AIDS were enrolled in a phase I/II protocol of recombinant CD4-IgG (rCD4-IgG) treatment. Patients’ peripheral blood leukocytes (PBL) were tested before, during, and after therapy with rCD4-IgG for T helper (TH) cell function assessed by antigen- and mitogen- stimulated proliferation and interleukin-2 production in response to influenza A virus, allogeneic PBL (alloantigens), and phytohemagglutinin. Although clinical benefit was not evident, rCD4-lgG treatment was associated with rapid and potent improved TH cell function for two of three stimuli tested in 90% of the patients. These data are complemented by an in vitro experimental model that demonstrates the opposing immunologic effects ofrgp120 and rCD4-lgG on TH cell function of PBL from uninfected individuals. Thus, restoration of TH cell function by rCD4-lgG in the absence ofincreased CD4 cell counts could be due to removal ofan immunosuppressive factor, possibly gp120. These findings suggest that rCD4-lgG can induce partial restoration of immune function in AIDS patients, even in the absence of apparent short-term clinical benefit.
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U2 - 10.1093/infdis/168.4.1012
DO - 10.1093/infdis/168.4.1012
M3 - Article
C2 - 8376811
AN - SCOPUS:0027291349
SN - 0022-1899
VL - 168
SP - 1012
EP - 1016
JO - Journal of Infectious Diseases
JF - Journal of Infectious Diseases
IS - 4
ER -