TY - JOUR
T1 - Effect of 3T3 plasma membranes on cells exposed to epidermal growth factor
AU - Lieberman, Michael A.
AU - Rothenberg, Paul
AU - Raben, Daniel M.
AU - Glaser, Luis
N1 - Funding Information:
We are grateful to Dr. C.F. Fox for sending us a culture of NR-6 cells and for allowing us to see his manuscript prior to publication. MAL was supported in part by a grant from the Damon Runyon-Walter Winchell Cancer Fund and by Grant No. GM 07157-04 from the NIH. PR was supported by Grant No. TO-5 GM 02016, and DMR by Grant No. GM 07067, both from the NIH. The work was supported by research grants GM 18405 from NIH and PCM 77 15972 from the National Science Foundation.
PY - 1980/1/29
Y1 - 1980/1/29
N2 - Epidermal growth factor (EGF) induced DNA synthesis in non-confluent, G0-arrested Swiss 3T3 fibroblasts is partially blocked by plasma membranes isolated from the EGF receptor deficient NR-6 Swiss 3T3 cell line. This inhibition could be due to either a steric block of the receptor by the membranes, a membrane induced down regulation of the EGF receptor, or a signal generated by membrane binding which is antagonistic towards the mitogenic signal generated by EGF. Binding measurements utilizing 125I-labeled EGF demonstrated that membranes do not block either the EGF induced down regulation of the receptor or alter the number of receptors on the surface. These results suggest that the membranes exert their inhibitory effect via generation of a signal which is antagonistic to the EGF induced mitogenic signal, with the result expressed as a reduced mitogenic response.
AB - Epidermal growth factor (EGF) induced DNA synthesis in non-confluent, G0-arrested Swiss 3T3 fibroblasts is partially blocked by plasma membranes isolated from the EGF receptor deficient NR-6 Swiss 3T3 cell line. This inhibition could be due to either a steric block of the receptor by the membranes, a membrane induced down regulation of the EGF receptor, or a signal generated by membrane binding which is antagonistic towards the mitogenic signal generated by EGF. Binding measurements utilizing 125I-labeled EGF demonstrated that membranes do not block either the EGF induced down regulation of the receptor or alter the number of receptors on the surface. These results suggest that the membranes exert their inhibitory effect via generation of a signal which is antagonistic to the EGF induced mitogenic signal, with the result expressed as a reduced mitogenic response.
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U2 - 10.1016/0006-291X(80)90389-7
DO - 10.1016/0006-291X(80)90389-7
M3 - Article
C2 - 6243947
AN - SCOPUS:0018848696
SN - 0006-291X
VL - 92
SP - 696
EP - 702
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 2
ER -