Effect of 3T3 plasma membranes on cells exposed to epidermal growth factor

Michael A. Lieberman, Paul Rothenberg, Daniel M. Raben, Luis Glaser

Research output: Contribution to journalArticle

Abstract

Epidermal growth factor (EGF) induced DNA synthesis in non-confluent, G0-arrested Swiss 3T3 fibroblasts is partially blocked by plasma membranes isolated from the EGF receptor deficient NR-6 Swiss 3T3 cell line. This inhibition could be due to either a steric block of the receptor by the membranes, a membrane induced down regulation of the EGF receptor, or a signal generated by membrane binding which is antagonistic towards the mitogenic signal generated by EGF. Binding measurements utilizing 125I-labeled EGF demonstrated that membranes do not block either the EGF induced down regulation of the receptor or alter the number of receptors on the surface. These results suggest that the membranes exert their inhibitory effect via generation of a signal which is antagonistic to the EGF induced mitogenic signal, with the result expressed as a reduced mitogenic response.

Original languageEnglish (US)
Pages (from-to)696-702
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume92
Issue number2
DOIs
StatePublished - Jan 29 1980
Externally publishedYes

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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