TY - JOUR
T1 - Effect of 2-Mercaptoethanol on Rabbit Pancreas Isoantibodies
AU - Brinckerhoff, Constance E.
AU - Rose, Noel R.
N1 - Funding Information:
These investigations were supported in part by USPHS Research Grant CA-05203 from Nat. Cancer Inst.
Funding Information:
Portions of this study are taken from a Dissertation presented by Constance E. Brinckerhoff to the Graduate School, State University of New York at Buffalo in partial fulfillment of the requirements for the Master's degree. Supported by USPHS Training Grant 5-T1-AI 130-07.
PY - 1967/1
Y1 - 1967/1
N2 - Sucrose density gradient ultra-centrifugation has shown that anti-rabbit pancreas rabbit isoantibodies could be classed with the 7S globulins. In agar electrophore-sis, the anti-rabbit pancreas rabbit antibody had a mobility of γ1 globulin. These antibodies were sensitive to reduction by 0.1 M mercaptoethanol. Reduction with mercaptoethanol and alkylation with iodoacetamide did not alter their sedimentation coefficient or precipitability by goat antiserum. There was, however, a total loss of complement fixation ability, and marked reduction in both passive cutaneous anaphylactic and precipitating abilities. The degree of reduction of these two abilities seemed to depend upon the height of the titer before treatment. The greater the titer, the more residual antibody after reduction. Naturally occurring antibodies to gastrointestinal mucosa also sedimented as 7S globulin and were inactivated by mercaptoethanol. 7S antibody to bovine serum albumin was mercaptoethanol resistant.
AB - Sucrose density gradient ultra-centrifugation has shown that anti-rabbit pancreas rabbit isoantibodies could be classed with the 7S globulins. In agar electrophore-sis, the anti-rabbit pancreas rabbit antibody had a mobility of γ1 globulin. These antibodies were sensitive to reduction by 0.1 M mercaptoethanol. Reduction with mercaptoethanol and alkylation with iodoacetamide did not alter their sedimentation coefficient or precipitability by goat antiserum. There was, however, a total loss of complement fixation ability, and marked reduction in both passive cutaneous anaphylactic and precipitating abilities. The degree of reduction of these two abilities seemed to depend upon the height of the titer before treatment. The greater the titer, the more residual antibody after reduction. Naturally occurring antibodies to gastrointestinal mucosa also sedimented as 7S globulin and were inactivated by mercaptoethanol. 7S antibody to bovine serum albumin was mercaptoethanol resistant.
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U2 - 10.3181/00379727-124-31716
DO - 10.3181/00379727-124-31716
M3 - Article
C2 - 6017779
AN - SCOPUS:0014046011
SN - 0037-9727
VL - 124
SP - 252
EP - 257
JO - Proceedings of the Society for Experimental Biology and Medicine
JF - Proceedings of the Society for Experimental Biology and Medicine
IS - 1
ER -