Effect of 2-Mercaptoethanol on Rabbit Pancreas Isoantibodies

Constance E. Brinckerhoff, Noel R. Rose

Research output: Contribution to journalArticlepeer-review

Abstract

Sucrose density gradient ultra-centrifugation has shown that anti-rabbit pancreas rabbit isoantibodies could be classed with the 7S globulins. In agar electrophore-sis, the anti-rabbit pancreas rabbit antibody had a mobility of γ1 globulin. These antibodies were sensitive to reduction by 0.1 M mercaptoethanol. Reduction with mercaptoethanol and alkylation with iodoacetamide did not alter their sedimentation coefficient or precipitability by goat antiserum. There was, however, a total loss of complement fixation ability, and marked reduction in both passive cutaneous anaphylactic and precipitating abilities. The degree of reduction of these two abilities seemed to depend upon the height of the titer before treatment. The greater the titer, the more residual antibody after reduction. Naturally occurring antibodies to gastrointestinal mucosa also sedimented as 7S globulin and were inactivated by mercaptoethanol. 7S antibody to bovine serum albumin was mercaptoethanol resistant.

Original languageEnglish (US)
Pages (from-to)252-257
Number of pages6
JournalProceedings of the Society for Experimental Biology and Medicine
Volume124
Issue number1
DOIs
StatePublished - Jan 1967

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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