Effect of ω-3 fatty acid (docosahexanoic acid) on BRCA1 gene expression and growth in MCF-7 cell line

S. K. Kachhap, P. P. Dange, R. H. Santani, S. S. Sawant, S. N. Ghosh

Research output: Contribution to journalArticle

Abstract

Recently, we have demonstrated that ω-6 fatty acid linoleic acid (LA) in presence of estradiol (E2) enhances proliferation and anchorage independent growth with down regulation of BRCA1 mRNA expression in MCF-7 cell line.18 Since ω-3 fatty acid (docosahexanoic acid, DHA) is known to block the promoting effect of ω-6 polyunsaturated fatty acid (LA), we wanted to see whether addition of DHA can inhibit the growth of MCF-7 cells which are exposed to LA + E2 and any alteration of BRCA1 mRNA expression could be seen in DHA treated culture. Experiments on MCF-7 cells with DHA revealed both decrease in proliferation and anchorage independency as compared to controls; while no change of BRCA1 mRNA expression was observed. Further, when DHA was administered to cells along with LA + E2, no change in BRCA1 expression was observed, however, a marked decrease in proliferation and soft agar colony formation was evident, indicating inhibition of MCF-7 cells following DHA treatment. Flow cytometric analysis showed that DHA treated cells either alone or in combination with LA + E2 induced marked G1/S and G2/M arrest of the cells, suggesting the inhibitory effect of DHA at this phase of cell cycle. However, neither typical DNA ladder nor fragmented nuclei or apoptotic bodies were observed, ruling out presence of apoptosis following DHA treatment.

Original languageEnglish (US)
Pages (from-to)257-263
Number of pages7
JournalCancer Biotherapy and Radiopharmaceuticals
Volume16
Issue number3
DOIs
StatePublished - Jan 1 2001
Externally publishedYes

ASJC Scopus subject areas

  • Oncology
  • Radiology Nuclear Medicine and imaging
  • Pharmacology
  • Cancer Research

Fingerprint Dive into the research topics of 'Effect of ω-3 fatty acid (docosahexanoic acid) on BRCA1 gene expression and growth in MCF-7 cell line'. Together they form a unique fingerprint.

  • Cite this