TY - JOUR
T1 - Effect of α-adrenergic blockade on arrhythmias induced byacute myocardial ischemia and reperfusion in the dog
AU - Bolli, Roberto
AU - Fisher, David J.
AU - Taylor, Addison A.
AU - Young, James B.
AU - Miller, Richard R.
N1 - Copyright:
Copyright 2018 Elsevier B.V., All rights reserved.
PY - 1984/12
Y1 - 1984/12
N2 - Studies in cats suggest α-adrenergic contributions to arrhythmiasduring myocardial ischemia and reperfusion. The validity of this concept in other species, however, remains uncertain. Thus, 106 chloralose-anesthetized open-chest dogs undergoing a 25 min coronary artery occlusion followed by reperfusion received saline (n=52), prazosin (1 mg/kg, n=26), phentolamine (5 mg/kg, n=18), or phentolamine (same dose)+propranolol (1 mg/kg, n=10). Alpha-blockade was confirmed by α-agonist dose-response studies. In phentolamine-treated dogs, arterial pressure and heart rate were kept constant to prevent exacerbation of ischemia. Control and treated groups were comparable with respect to variables known to affect arrhythmias, such as size of occluded and reperfused vascular beds, coronary collateral flow, severity of ischemia estimated from intramyocardial CO2 tension, and peak reactive hyperemia. During coronary occlusion, the number of single premature ventricular complexes was reduced by phentolamine (P<0.01), but not by prazosin or phentolamine+propranolol; no treatment affected the total number of couplets, ventricular tachycardia episodes and ventricular ectopic complexes, or the incidence of ventricular tachycardia and ventricular fibrillation. During coronary reperfusion, arrhythmias did not differ in control and treated groups. Thus, selective α1-(prazosin), nonselective α1- and α2-(phentolamine), and combined α- and β-blockade (phentolamine+propranolol) failed to attenuate complex arrhythmias induced by acute myocardial ischemia and reperfusion. Alpha-adrenergic mechanisms appear unimportant in the genesis of these arrhythmias in the canine model.
AB - Studies in cats suggest α-adrenergic contributions to arrhythmiasduring myocardial ischemia and reperfusion. The validity of this concept in other species, however, remains uncertain. Thus, 106 chloralose-anesthetized open-chest dogs undergoing a 25 min coronary artery occlusion followed by reperfusion received saline (n=52), prazosin (1 mg/kg, n=26), phentolamine (5 mg/kg, n=18), or phentolamine (same dose)+propranolol (1 mg/kg, n=10). Alpha-blockade was confirmed by α-agonist dose-response studies. In phentolamine-treated dogs, arterial pressure and heart rate were kept constant to prevent exacerbation of ischemia. Control and treated groups were comparable with respect to variables known to affect arrhythmias, such as size of occluded and reperfused vascular beds, coronary collateral flow, severity of ischemia estimated from intramyocardial CO2 tension, and peak reactive hyperemia. During coronary occlusion, the number of single premature ventricular complexes was reduced by phentolamine (P<0.01), but not by prazosin or phentolamine+propranolol; no treatment affected the total number of couplets, ventricular tachycardia episodes and ventricular ectopic complexes, or the incidence of ventricular tachycardia and ventricular fibrillation. During coronary reperfusion, arrhythmias did not differ in control and treated groups. Thus, selective α1-(prazosin), nonselective α1- and α2-(phentolamine), and combined α- and β-blockade (phentolamine+propranolol) failed to attenuate complex arrhythmias induced by acute myocardial ischemia and reperfusion. Alpha-adrenergic mechanisms appear unimportant in the genesis of these arrhythmias in the canine model.
KW - Acute myocardial ischemia
KW - Alpha-adrenergic blockade
KW - Alpha-adrenergic receptors
KW - Arrhythmias
KW - Coronary artery occlusion
KW - Coronary artery reperfusion
KW - Phentolamine
KW - Prazosin
KW - Propranolol
UR - http://www.scopus.com/inward/record.url?scp=0021637480&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0021637480&partnerID=8YFLogxK
U2 - 10.1016/S0022-2828(84)80037-1
DO - 10.1016/S0022-2828(84)80037-1
M3 - Article
C2 - 6152473
AN - SCOPUS:0021637480
SN - 0022-2828
VL - 16
SP - 1101
EP - 1117
JO - Journal of Molecular and Cellular Cardiology
JF - Journal of Molecular and Cellular Cardiology
IS - 12
ER -