Abstract
The increase in the prevalence and impact of infections caused by carbapenemase-producing Enterobacteriaceae is a global health concern. Therefore, rapid and accurate methods to detect these organisms in any clinical microbiology laboratory, including those in resource-limited settings, are essential to prevent and contain their spread. It is also important to differentiate between serine- and metaldependent carbapenemases elaborated by carbapenemase-producing isolates for epidemiologic, infection control and prevention, and therapeutic purposes. Here, we describe the development and evaluation of the EDTA-modified carbapenem inactivation method (eCIM), an assay for discriminating between serine- and metaldependent (i.e., metallo-β-lactamases [MBLs]) carbapenemases when used in conjunction with the modified carbapenem inactivation method (mCIM). The eCIM had an overall sensitivity and specificity of 100% and was adopted by the Clinical and Laboratory Standards Institute as a method to use in combination with the mCIM to identify MBL-producing Enterobacteriaceae.
Original language | English (US) |
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Article number | e01757-18 |
Journal | Journal of clinical microbiology |
Volume | 57 |
Issue number | 5 |
DOIs | |
State | Published - May 2019 |
Keywords
- Carbapenemase
- Carbapenemase-producing Enterobacteriaceae
- ECIM
- EDTA-modified carbapenem inactivation method
- MBL
- MCIM
- Metallo-β-lactamase
- Modified carbapenem inactivation method
- Phenotypic detection
ASJC Scopus subject areas
- Microbiology (medical)