EBV antigens in lymphocytes of patients with exudative tonsillitis, infectious mononucleosis and Hodgkin's disease

R. W. Veltri, J. E. McClung, P. M. Sprinkle

Research output: Contribution to journalArticle

Abstract

A protocol utilizing isokinetic gradients to isolate human lymphocytes is combined with another that purifies the C3 receptor-bearing B lymphocyte subpopulation thus enriching the EB virus genome-carrying population. Also, rabbit antisera were prepared to the Epstein-Barr virus nuclear antigen (EBNA) and the early antigen (EA) and utilized in an indirect immunofluorescence test (IIT) to detect these antigens in human lymphocytes isolated fom various disease states. Using these methods we demonstrated excellent correlation between standard methods previously employed to detect EB virus-coded antigens and our IIT employing xenogenic antisera. Such tests were done on lymphoblastoid cell lines as well as lymphocytes isolated directly from patients with EB virus lymphoproliferative diseases. Human palatine tonsil-derived lymphocytes from children with exudative tonsillitis and peripheral blood lymphocytes of infectious mononucleosis contained only EBNA in C3 receptor-bearing B lymphocytes. However, patients with lymphoproliferative disorders, including Hodgkin's disease, harbored in their spleens and lymph nodes lymphocytes producing both EBNA and EA.

Original languageEnglish (US)
Pages (from-to)683-687
Number of pages5
JournalInternational Journal of Cancer
Volume21
Issue number6
Publication statusPublished - 1978
Externally publishedYes

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ASJC Scopus subject areas

  • Cancer Research
  • Oncology

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