A protocol utilizing isokinetic gradients to isolate human lymphocytes is combined with another that purifies the C3 receptor‐bearing B lymphocyte subpopulation, thus enriching the EB virus genome‐carrying population. Also, rabbit antisera were prepared to the Epstein‐Barr virus nuclear antigen (EBNA) and the early antigen (EA) and utilized in an indirect immunofluorescence test (IIT) to detect these antigens in human lymphocytes isolated from various disease states. Using these methods we demonstrated excellent correlation between standard methods previously employed to detect EB virus‐coded antigens and our IIT employing xenogenic antisera. Such tests were done on lymphoblastoid cell lines as well as lymphocytes isolated directly from patients with EB vims lymphoproliferative diseases. Human palatine tonsil‐derived lymphocytes from children with exudative tonsillitis and peripheral blood lymphocytes of infectious mononucleosis contained only EBNA in C3 receptor‐bearing B lymphocytes. However, patients with lymphoproliferative disorders, including Hodgkin's disease, harbored in their spleens and lymph nodes lymphocytes producing both EBNA and EA.
ASJC Scopus subject areas
- Cancer Research