Early versus late growth S. aureus adhesion to immobilized platelets under physiological shear regimes

Niraj P.E. George, Konstantinos Konstantopoulos, Julia M. Ross

Research output: Contribution to conferencePaperpeer-review

Abstract

Staphylococcus aureus causes a wide range of infections in humans. Infection is initiated by the adhesion of surface proteins on S. aureus to host extracellular matrix proteins including fibronectin (Fn) and fibrinogen (Fg). Since platelets can bind Fn and Fg via the αIIBβ 3 receptor, the molecular mechanisms of infection and thrombosis are likely to be intertwined. S. aureus is known to bind Fn through the adhesins FnbpA and FnbpB. FnbpA has also been shown to interact with Fg. In addition, our previous studies have shown that Fg can mediate S. aureus - immobilized platelet interactions via the adhesins ClfA and SdrCDE. While S. aureus adhesins ClfA and SdrCDE are expressed in the late exponential growth phase, fibronectin binding proteins (Fnbps) are expressed in early exponential growth. Hence staphylococci could use alternate mechanisms to adhere to immobilized platelets depending on their growth phase. In this study, we demonstrate shear dependant adhesion of S. aureus to platelets via Fnbps both in the presence and absence of exogenously added fibrinogen and fibronectin. At all shear rates, a significant decrease in adhesion is reported with Fnbp negative mutant in the absence of exogenously added fibronectin. The above findings may lead to the development of novel therapeutics and preventive approaches against staphylococcal infections.

Original languageEnglish (US)
Number of pages1
StatePublished - Dec 1 2005
Externally publishedYes
Event05AIChE: 2005 AIChE Annual Meeting and Fall Showcase - Cincinnati, OH, United States
Duration: Oct 30 2005Nov 4 2005

Other

Other05AIChE: 2005 AIChE Annual Meeting and Fall Showcase
Country/TerritoryUnited States
CityCincinnati, OH
Period10/30/0511/4/05

ASJC Scopus subject areas

  • Engineering(all)

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