Early treatment of transient focal cerebral ischemia with bovine PEGylated carboxy hemoglobin transfusion

Judith A. Klaus, Kathleen K. Kibler, Abraham Abuchowski, Raymond C Koehler

Research output: Contribution to journalArticle

Abstract

The effect of transfusion of PEGylated hemoglobin (PEG-Hb) was evaluated in anesthetized rats subjected to 2 hours of focal cerebral ischemia and 1 day of reperfusion. PEG-Hb was stored in the carboxy state (PEG-COHb) to reduce autooxidation and increase the shelf life. Transfusion of 10 ml/kg of PEG-COHb at 20 minutes of ischemia did not alter arterial blood pressure or increase red cell flux in the ischemic core. Plasma hemoglobin increased to only 0.6 g/dL, yet infarct volume was markedly decreased and neurological deficits were improved. We conclude that early topload transfusion of PEG-COHb protects the brain from ischemic stroke.

Original languageEnglish (US)
Pages (from-to)223-229
Number of pages7
JournalArtificial Cells, Blood Substitutes, and Biotechnology
Volume38
Issue number5
DOIs
StatePublished - Oct 2010

Fingerprint

Hemoglobin
Transient Ischemic Attack
Polyethylene glycols
Hemoglobins
Blood pressure
Brain Ischemia
Reperfusion
Rats
Brain
Arterial Pressure
Ischemia
Stroke
Cells
Fluxes
Plasmas

Keywords

  • Blood substitute
  • Carbon monoxide
  • Focal cerebral ischemia
  • PEGylated hemoglobin
  • Stroke

ASJC Scopus subject areas

  • Biomedical Engineering
  • Biotechnology

Cite this

Early treatment of transient focal cerebral ischemia with bovine PEGylated carboxy hemoglobin transfusion. / Klaus, Judith A.; Kibler, Kathleen K.; Abuchowski, Abraham; Koehler, Raymond C.

In: Artificial Cells, Blood Substitutes, and Biotechnology, Vol. 38, No. 5, 10.2010, p. 223-229.

Research output: Contribution to journalArticle

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