Early treatment of transient focal cerebral ischemia with bovine PEGylated carboxy hemoglobin transfusion

Judith A. Klaus, Kathleen K. Kibler, Abraham Abuchowski, Raymond C. Koehler

Research output: Contribution to journalArticle

Abstract

The effect of transfusion of PEGylated hemoglobin (PEG-Hb) was evaluated in anesthetized rats subjected to 2 hours of focal cerebral ischemia and 1 day of reperfusion. PEG-Hb was stored in the carboxy state (PEG-COHb) to reduce autooxidation and increase the shelf life. Transfusion of 10 ml/kg of PEG-COHb at 20 minutes of ischemia did not alter arterial blood pressure or increase red cell flux in the ischemic core. Plasma hemoglobin increased to only 0.6 g/dL, yet infarct volume was markedly decreased and neurological deficits were improved. We conclude that early topload transfusion of PEG-COHb protects the brain from ischemic stroke.

Original languageEnglish (US)
Pages (from-to)223-229
Number of pages7
JournalArtificial Cells, Blood Substitutes, and Biotechnology
Volume38
Issue number5
DOIs
StatePublished - Oct 1 2010

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Keywords

  • Blood substitute
  • Carbon monoxide
  • Focal cerebral ischemia
  • PEGylated hemoglobin
  • Stroke

ASJC Scopus subject areas

  • Biotechnology
  • Biomedical Engineering

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