Early stimulation of phospholipid methylation in Xenopus oocytes by progesterone

Francois Godeau, Teruko Ishizaka, S. S. Koide

Research output: Contribution to journalArticlepeer-review

Abstract

Progesterone induced a transient increase in the incorporation of [3H]methyl groups into phospholipids of Xenopus oocytes followed by a rise in 45Ca2+ uptake. Phospholipid methylation reached a maximum as early as 15 s after progesterone treatment and returned to basal level within 2 min. Steroids inactive in promoting oocyte maturation were less effective in affecting phospholipid methylation. Methyltransferase inhibitors, 3-deaza-SIBA, SIBA, and Sinefungin, inhibited progesterone-activated stimulation of phospholipid methylation, calcium uptake and meiotic maturation. Phospholipid methylation is the earliest detectable biochemical event occurring in oocytes after exposure to progesterone followed by calcium influx and leading to germinal vesicle dissolution.

Original languageEnglish (US)
Pages (from-to)35-41
Number of pages7
JournalCell Differentiation
Volume16
Issue number1
DOIs
StatePublished - Feb 1985

Keywords

  • Xenopus
  • oocyte maturation
  • phospholipid-methylation
  • progesterone

ASJC Scopus subject areas

  • Developmental Biology

Fingerprint Dive into the research topics of 'Early stimulation of phospholipid methylation in Xenopus oocytes by progesterone'. Together they form a unique fingerprint.

Cite this