Early-life gut microbiome composition and milk allergy resolution

Supinda Bunyavanich, Nan Shen, Alexander Grishin, Robert Wood, Wesley Burks, Peter Dawson, Stacie M. Jones, Donald Y.M. Leung, Hugh Sampson, Scott Sicherer, Jose C. Clemente

Research output: Contribution to journalArticle

Abstract

Background Gut microbiota may play a role in the natural history of cow's milk allergy. Objective We sought to examine the association between early-life gut microbiota and the resolution of cow's milk allergy. Methods We studied 226 children with milk allergy who were enrolled at infancy in the Consortium of Food Allergy observational study of food allergy. Fecal samples were collected at age 3 to 16 months, and the children were followed longitudinally with clinical evaluation, milk-specific IgE levels, and milk skin prick test performed at enrollment, 6 months, 12 months, and yearly thereafter up until age 8 years. Gut microbiome was profiled by 16s rRNA sequencing and microbiome analyses performed using Quantitative Insights into Microbial Ecology (QIIME), Phylogenetic Investigation of Communities by Reconstruction of Unobserved States (PICRUSt), and Statistical Analysis of Metagenomic Profiles (STAMP). Results Milk allergy resolved by age 8 years in 128 (56.6%) of the 226 children. Gut microbiome composition at age 3 to 6 months was associated with milk allergy resolution by age 8 years (PERMANOVA P = .047), with enrichment of Clostridia and Firmicutes in the infant gut microbiome of subjects whose milk allergy resolved. Metagenome functional prediction supported decreased fatty acid metabolism in the gut microbiome of subjects whose milk allergy resolved (η2 = 0.43; ANOVA P = .034). Conclusions Early infancy is a window during which gut microbiota may shape food allergy outcomes in childhood. Bacterial taxa within Clostridia and Firmicutes could be studied as probiotic candidates for milk allergy therapy.

Original languageEnglish (US)
Pages (from-to)1122-1130
Number of pages9
JournalJournal of Allergy and Clinical Immunology
Volume138
Issue number4
DOIs
StatePublished - Oct 1 2016

Keywords

  • 16s rRNA sequencing
  • Bacteroidetes
  • Clostridia
  • Cow's milk allergy
  • Firmicutes
  • fatty acid
  • food allergy
  • metagenome
  • microbiome
  • microbiota

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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  • Cite this

    Bunyavanich, S., Shen, N., Grishin, A., Wood, R., Burks, W., Dawson, P., Jones, S. M., Leung, D. Y. M., Sampson, H., Sicherer, S., & Clemente, J. C. (2016). Early-life gut microbiome composition and milk allergy resolution. Journal of Allergy and Clinical Immunology, 138(4), 1122-1130. https://doi.org/10.1016/j.jaci.2016.03.041