Early KLRG1+ but Not CD57+CD8+ T Cells in Primary Cytomegalovirus Infection Predict Effector Function and Viral Control

Aki Hoji, Iulia D. Popescu, Matthew R. Pipeling, Pali Dedhiya Shah, Spencer A. Winters, John F. McDyer

Research output: Contribution to journalArticle

Abstract

CMV remains an important opportunistic pathogen in high-risk lung transplant recipients. We characterized the phenotype and function of CD8+ T cells from acute/primary into chronic CMV infection in 23 (donor+/recipient-; D+R-) lung transplant recipients and found rapid induction of both KLRG1+ and/or CD57+ CMV-specific CD8+ T cells with unexpected coexpression of CD27. These cells demonstrated maturation from an acute effector T cell (TAEFF) to an effector memory T cell (TEM) phenotype with progressive enrichment of KLRG1+CD57+CD27- cells into memory. CMV-specific KLRG1+ TAEFF were capable of in vitro proliferation that diminished upon acquisition of CD57, whereas only KLRG1+ expression correlated with T-bet expression and effector function. In contrast to blood TAEFF, lung mucosal TAEFF demonstrated reduced KLRG1/T-bet expression but similar CD57 levels. Additionally, increased KLRG1+TAEFF were associated with early immune viral control following primary infection. To our knowledge, our findings provide new insights into the roles of KLRG1 and CD57 expression in human T cells, forming the basis for a refined model of CD8+ T cell differentiation during CMV infection.

Original languageEnglish (US)
Pages (from-to)2063-2075
Number of pages13
JournalJournal of immunology (Baltimore, Md. : 1950)
Volume203
Issue number8
DOIs
StatePublished - Oct 15 2019

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Cytomegalovirus Infections
T-Lymphocytes
Lung
Infection
Phenotype
Cell Differentiation
Tissue Donors

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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Early KLRG1+ but Not CD57+CD8+ T Cells in Primary Cytomegalovirus Infection Predict Effector Function and Viral Control. / Hoji, Aki; Popescu, Iulia D.; Pipeling, Matthew R.; Shah, Pali Dedhiya; Winters, Spencer A.; McDyer, John F.

In: Journal of immunology (Baltimore, Md. : 1950), Vol. 203, No. 8, 15.10.2019, p. 2063-2075.

Research output: Contribution to journalArticle

Hoji, Aki ; Popescu, Iulia D. ; Pipeling, Matthew R. ; Shah, Pali Dedhiya ; Winters, Spencer A. ; McDyer, John F. / Early KLRG1+ but Not CD57+CD8+ T Cells in Primary Cytomegalovirus Infection Predict Effector Function and Viral Control. In: Journal of immunology (Baltimore, Md. : 1950). 2019 ; Vol. 203, No. 8. pp. 2063-2075.
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