TY - JOUR
T1 - Early chemokine expression induced by interferon-gamma in a murine model of Hashimoto's thyroiditis
AU - Kimura, Hiroaki
AU - Kimura, Miho
AU - Rose, Noel R.
AU - Caturegli, Patrizio
N1 - Funding Information:
This work was supported by grant RO1-DK55670 from the National Institutes of Health (NIDDK) to P.C.
PY - 2004/12
Y1 - 2004/12
N2 - Chemokines represent a group of small, secreted proteins mainly involved in navigating leukocytes towards site of inflammation. Some chemokines have been implicated in the pathogenesis of autoimmune diseases, which are characterized by an ectopic retention of leukocytes within the target organ, ultimately leading to loss of function. To determine the chemokines profile expressed in the thyroid gland upon chronic exposure to interferon-gamma (IFNγ), we analyzed C57BL6 transgenic mice that aberrantly express IFNγ under control of the thyroglobulin promoter. We compared by reverse transcriptase PCR the thyroidal expression of 10 chemokines (CCL1 through 5 and CXCL9 through 13) in thyr-IFNγ transgenics and wild-type littermates. We found that transgenics exclusively expressed CCL4, CXCL9, and CXCL11, and showed increased expression of CCL5 and CXCL10. This chemokine profile was associated with moderate mononuclear cell infiltration of the thyroid stroma that, however, decreased significantly after 2 months of age and did not organize into lymphoid structures. Our findings indicate that the isolated expression of IFNγ is capable of recruiting mononuclear cells but they do not progress to full lymphoid transformation of the thyroid.
AB - Chemokines represent a group of small, secreted proteins mainly involved in navigating leukocytes towards site of inflammation. Some chemokines have been implicated in the pathogenesis of autoimmune diseases, which are characterized by an ectopic retention of leukocytes within the target organ, ultimately leading to loss of function. To determine the chemokines profile expressed in the thyroid gland upon chronic exposure to interferon-gamma (IFNγ), we analyzed C57BL6 transgenic mice that aberrantly express IFNγ under control of the thyroglobulin promoter. We compared by reverse transcriptase PCR the thyroidal expression of 10 chemokines (CCL1 through 5 and CXCL9 through 13) in thyr-IFNγ transgenics and wild-type littermates. We found that transgenics exclusively expressed CCL4, CXCL9, and CXCL11, and showed increased expression of CCL5 and CXCL10. This chemokine profile was associated with moderate mononuclear cell infiltration of the thyroid stroma that, however, decreased significantly after 2 months of age and did not organize into lymphoid structures. Our findings indicate that the isolated expression of IFNγ is capable of recruiting mononuclear cells but they do not progress to full lymphoid transformation of the thyroid.
KW - Chemokine
KW - Hashimoto's thyroiditis
KW - Murine
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U2 - 10.1016/j.yexmp.2004.08.004
DO - 10.1016/j.yexmp.2004.08.004
M3 - Article
C2 - 15507231
AN - SCOPUS:7044254634
SN - 0014-4800
VL - 77
SP - 161
EP - 167
JO - Experimental and Molecular Pathology
JF - Experimental and Molecular Pathology
IS - 3
ER -