Early chemokine expression induced by interferon-gamma in a murine model of Hashimoto's thyroiditis

Hiroaki Kimura, Miho Kimura, Noel R. Rose, Patrizio Caturegli

Research output: Contribution to journalArticlepeer-review

Abstract

Chemokines represent a group of small, secreted proteins mainly involved in navigating leukocytes towards site of inflammation. Some chemokines have been implicated in the pathogenesis of autoimmune diseases, which are characterized by an ectopic retention of leukocytes within the target organ, ultimately leading to loss of function. To determine the chemokines profile expressed in the thyroid gland upon chronic exposure to interferon-gamma (IFNγ), we analyzed C57BL6 transgenic mice that aberrantly express IFNγ under control of the thyroglobulin promoter. We compared by reverse transcriptase PCR the thyroidal expression of 10 chemokines (CCL1 through 5 and CXCL9 through 13) in thyr-IFNγ transgenics and wild-type littermates. We found that transgenics exclusively expressed CCL4, CXCL9, and CXCL11, and showed increased expression of CCL5 and CXCL10. This chemokine profile was associated with moderate mononuclear cell infiltration of the thyroid stroma that, however, decreased significantly after 2 months of age and did not organize into lymphoid structures. Our findings indicate that the isolated expression of IFNγ is capable of recruiting mononuclear cells but they do not progress to full lymphoid transformation of the thyroid.

Original languageEnglish (US)
Pages (from-to)161-167
Number of pages7
JournalExperimental and Molecular Pathology
Volume77
Issue number3
DOIs
StatePublished - Dec 2004

Keywords

  • Chemokine
  • Hashimoto's thyroiditis
  • Murine

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Molecular Biology
  • Clinical Biochemistry

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