Early and extensive contribution of pericytes/vascular smooth muscle cells to microvascular proliferation in glioblastoma multiforme

An immuno-light and immuno-electron microscopic study

Pieter Wesseling, Reinier O. Schlingemann, Frank J R Rietveld, Monique Link, Peter C. Burger, Dirk J. Ruiter

Research output: Contribution to journalArticle

Abstract

Although florid microvascular proliferation (MVP) in glioblastoma multiforme (GBM) has long been considered as proliferation of endothelial cells (EC), recent immuno-light microscopic studies demonstrated many a-smooth muscle actin (a-sm actin)-positive cells in this MVP, suggesting a major contribution of pericytes and/or vascular smooth muscle cells (VSMC). Under certain culture conditions, however, a-sm actin expression has also been described in EC. In order to further investigate to what extent pericytes/VSMC participate in MVP in GBM, we performed an immunohistochemical study at both the light and electron microscopic levels with anti-a-sm actin, with an antibody against EC (EN-4) and with an antibody recently described to react with “activated” pericytes in conditions with neovascularization (anti-high molecular weight-melanoma associated antigen). In this detailed study of MVP in GBM, two distinct cell types could be recognized on the basis of a consistent ultrastructural localization and immunophenotype: EC and pericytes/VSMC; no transitional forms were found between these two cell types. The contribution of pericytes/VSMC to MVP in GBM was extensive and already present in many delicate tumor capillaries, suggesting not only an essential but also an early role of these cells in this type of tumor angiogenesis.

Original languageEnglish (US)
Pages (from-to)304-310
Number of pages7
JournalJournal of Neuropathology and Experimental Neurology
Volume54
Issue number3
StatePublished - 1995

Fingerprint

Pericytes
Glioblastoma
Vascular Smooth Muscle
Smooth Muscle Myocytes
Electrons
Light
Endothelial Cells
Smooth Muscle
Actins
Antibodies
Neoplasms

Keywords

  • Endothelium
  • Glioblastoma multiforme
  • Immuno-electron microscopy
  • Neovascularization
  • Pericyte
  • Vascular smooth muscle

ASJC Scopus subject areas

  • Clinical Neurology
  • Pathology and Forensic Medicine
  • Cellular and Molecular Neuroscience
  • Neurology
  • Neuroscience(all)

Cite this

Early and extensive contribution of pericytes/vascular smooth muscle cells to microvascular proliferation in glioblastoma multiforme : An immuno-light and immuno-electron microscopic study. / Wesseling, Pieter; Schlingemann, Reinier O.; Rietveld, Frank J R; Link, Monique; Burger, Peter C.; Ruiter, Dirk J.

In: Journal of Neuropathology and Experimental Neurology, Vol. 54, No. 3, 1995, p. 304-310.

Research output: Contribution to journalArticle

Wesseling, Pieter ; Schlingemann, Reinier O. ; Rietveld, Frank J R ; Link, Monique ; Burger, Peter C. ; Ruiter, Dirk J. / Early and extensive contribution of pericytes/vascular smooth muscle cells to microvascular proliferation in glioblastoma multiforme : An immuno-light and immuno-electron microscopic study. In: Journal of Neuropathology and Experimental Neurology. 1995 ; Vol. 54, No. 3. pp. 304-310.
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