E-selectin and very late activation antigen-4 mediate adhesion of hematopoietic progenitor cells to bone marrow endothelium

P. M L Rood, M. W. Dercksen, H. Cazemier, J. M. Kerst, A. E G K Von dem Borne, W. R. Gerritsen, C. E. Van der Schoot

Research output: Contribution to journalArticle

Abstract

Adhesion of CD34 + hematopoietic progenitor cells (HPCs) to sinusoidal endothelium probably plays a key role in homing of transplanted CD34+ HPCs to the bone marrow (BM). We have investigated the role of various adhesion molecules in the interaction of purified CD34+ HPCs derived from BM or peripheral blood (PB) and a human BM-derived endothelial cell line. Adhesion of CD34+ HPCs to endothelial cells was measured with the use of a double-color flow microfluorimetric adhesion assay. In this assay, adhesion is measured under stirring conditions, simulating blood flow in sinusoidal marrow vessels. Adhesion of PB CD34+ cells to human BM endothelial cells (HBMECs) was observed only after interleukin (IL)-1β prestimulation of the endothelial cells. This adhesion was strongly increased after addition of phorbol-myristate acetate (PMA). Adhesion of PB CD34+ cells to IL-1β-prestimulated HBMECs was inhibited by blocking monoclonal antibodies (mAbs) against E-selectin and by neuraminidase treatment of the PB CD34+ cells. mAbs against very late activation antigen (VLA)-4 inhibited adhesion only when the E-selectin-mediated interaction was prevented. No clear inhibiting effect was found with blocking mAbs against β2-integrins. Stimulation with the β1-integrin-activating mAb, 8A2, induced adhesion of CD34+ cells to endothelial cells. In conclusion, stimulation of both endothelial cells and CD34+ HPCs is necessary for adhesion of CD34+ HPCs to endothelial cells. We furthermore demonstrated that E-selectin and VLA-4 mediated this adhesion.

Original languageEnglish (US)
Pages (from-to)477-484
Number of pages8
JournalAnnals of Hematology
Volume79
Issue number9
StatePublished - 2000
Externally publishedYes

Fingerprint

Integrin alpha4beta1
E-Selectin
Hematopoietic Stem Cells
Endothelium
Endothelial Cells
Bone Marrow
Blood Cells
Blocking Antibodies
Monoclonal Antibodies
Interleukin-1
Integrins
Neuraminidase
Tetradecanoylphorbol Acetate
Cell Adhesion
Bone Marrow Cells
Color
Cell Line

Keywords

  • Adhesion
  • Bone marrow endothelium
  • CD34 hematopoietic progenitor cells
  • E-selectin
  • VLA-4

ASJC Scopus subject areas

  • Hematology

Cite this

Rood, P. M. L., Dercksen, M. W., Cazemier, H., Kerst, J. M., Von dem Borne, A. E. G. K., Gerritsen, W. R., & Van der Schoot, C. E. (2000). E-selectin and very late activation antigen-4 mediate adhesion of hematopoietic progenitor cells to bone marrow endothelium. Annals of Hematology, 79(9), 477-484.

E-selectin and very late activation antigen-4 mediate adhesion of hematopoietic progenitor cells to bone marrow endothelium. / Rood, P. M L; Dercksen, M. W.; Cazemier, H.; Kerst, J. M.; Von dem Borne, A. E G K; Gerritsen, W. R.; Van der Schoot, C. E.

In: Annals of Hematology, Vol. 79, No. 9, 2000, p. 477-484.

Research output: Contribution to journalArticle

Rood, PML, Dercksen, MW, Cazemier, H, Kerst, JM, Von dem Borne, AEGK, Gerritsen, WR & Van der Schoot, CE 2000, 'E-selectin and very late activation antigen-4 mediate adhesion of hematopoietic progenitor cells to bone marrow endothelium', Annals of Hematology, vol. 79, no. 9, pp. 477-484.
Rood PML, Dercksen MW, Cazemier H, Kerst JM, Von dem Borne AEGK, Gerritsen WR et al. E-selectin and very late activation antigen-4 mediate adhesion of hematopoietic progenitor cells to bone marrow endothelium. Annals of Hematology. 2000;79(9):477-484.
Rood, P. M L ; Dercksen, M. W. ; Cazemier, H. ; Kerst, J. M. ; Von dem Borne, A. E G K ; Gerritsen, W. R. ; Van der Schoot, C. E. / E-selectin and very late activation antigen-4 mediate adhesion of hematopoietic progenitor cells to bone marrow endothelium. In: Annals of Hematology. 2000 ; Vol. 79, No. 9. pp. 477-484.
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AU - Rood, P. M L

AU - Dercksen, M. W.

AU - Cazemier, H.

AU - Kerst, J. M.

AU - Von dem Borne, A. E G K

AU - Gerritsen, W. R.

AU - Van der Schoot, C. E.

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