E. coli recA protein-directed cleavage of phage λ repressor requires polynucleotide

Nancy L. Craig, Jeffrey W. Roberts

Research output: Contribution to journalArticle

Abstract

The recA protein mediates both genetic recombination and several cellular responses to DNA damage,including the induction of temperate bacteriophage. Induction of phage λ results from proteolytic cleavage of λ repressor directed by recA protein. We show here that this cleavage reaction requires both polynucleotide and ATP. We suggest that a stoichiometric complex of recA protein and DNA is active both to destroy repressors by proteolytic cleavage and to initiate pairing of this DNA to its homologous sequence in a DNA duplex ('strand invasion').

Original languageEnglish (US)
Pages (from-to)26-30
Number of pages5
JournalNature
Volume283
Issue number5742
DOIs
StatePublished - Dec 1 1980
Externally publishedYes

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ASJC Scopus subject areas

  • General

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