Abstract
The recA protein mediates both genetic recombination and several cellular responses to DNA damage,including the induction of temperate bacteriophage. Induction of phage λ results from proteolytic cleavage of λ repressor directed by recA protein. We show here that this cleavage reaction requires both polynucleotide and ATP. We suggest that a stoichiometric complex of recA protein and DNA is active both to destroy repressors by proteolytic cleavage and to initiate pairing of this DNA to its homologous sequence in a DNA duplex ('strand invasion').
Original language | English (US) |
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Pages (from-to) | 26-30 |
Number of pages | 5 |
Journal | Nature |
Volume | 283 |
Issue number | 5742 |
DOIs | |
State | Published - Dec 1 1980 |
Externally published | Yes |
ASJC Scopus subject areas
- General