@article{9e44ad3b3f4448fcac8101699a0a1b12,
title = "Dystroglycan Organizes Axon Guidance Cue Localization and Axonal Pathfinding",
abstract = "Precise patterning of axon guidance cue distribution is critical for nervous system development. Using a murine forward genetic screen for novel determinants of axon guidance, we identified B3gnt1 and ISPD as required for the glycosylation of dystroglycan in vivo. Analysis of B3gnt1, ISPD, and dystroglycan mutant mice revealed a critical role for glycosylated dystroglycan in the development of several longitudinal axon tracts. Remarkably, the axonal guidance defects observed in B3gnt1, ISPD, and dystroglycan mutants resemble several of the axon guidance defects found in mice lacking the axon guidance cue Slit and its receptor Robo. This similarity is explained by our observations that dystroglycan binds directly to Slit and is required for proper Slit localization within the basement membrane and floor plate in vivo. These findings establish a novel role for glycosylated dystroglycan as a key determinant of axon guidance cue distribution and function in the mammalian nervous system.",
author = "Wright, {Kevin M.} and Lyon, {Krissy A.} and Haiwen Leung and Leahy, {Daniel J.} and Le Ma and Ginty, {David D.}",
note = "Funding Information: We thank members of the Ginty laboratory for assistance and discussions throughout the course of this project, Christopher Walsh and Chiara Manzini for insightful comments, and Randal Hand, Alex Kolodkin, Seth Margolis, Martin Riccomagno, Sarah Sarsfield, and Megan Straiko for comments on the manuscript. We thank Kevin Campbell and Alain Chedotal for providing DNA constructs and Camille Charoy and Valerie Castellani for assistance with spinal cord open-book preparations. The 2H3 and Nestin monoclonal antibodies were obtained from the Developmental Studies Hybridoma Bank developed under the auspices of the NICHD and maintained by The University of Iowa, Department of Biology, Iowa City, IA 52242. This work was supported by NIH grants NS34814 (D.D.G.), NS062047 (L.M.), and R01HD055545 (D.J.L.), and the Johns Hopkins Brain Sciences Institute (D.D.G.). D.D.G. is an investigator of the Howard Hughes Medical Institute. ",
year = "2012",
month = dec,
day = "6",
doi = "10.1016/j.neuron.2012.10.009",
language = "English (US)",
volume = "76",
pages = "931--944",
journal = "Neuron",
issn = "0896-6273",
publisher = "Cell Press",
number = "5",
}