Dysregulation in IL-12 secretion by neutrophils from HIV-infected patients

A. Vecchiarelli, C. Monari, B. Palazzetti, F. Bistoni, Arturo Casadevall

Research output: Contribution to journalArticle

Abstract

It is generally believed that neutrophils from HIV-infected patients are functionally competent, but several studies have shown impairment in neutrophil fungal killing and cytokine production. In this study we evaluated the ability of neutrophils from healthy donors and HIV-infected patients to produce IL-12 in response to stimulation with Candida albicans, lipopolysaccharide (LPS) and Cryptococcus neoformans (acapsular and encapsulated), with and without MoAb opsonization. Neutrophils from healthy donors secreted IL-12 in response to LPS or C. albicans but not in response to encapsulated or acapsular C. neoformans, regardless of MoAb opsonization. Surprisingly, neutrophils from HIV-infected patients demonstrated constitutive IL-12 production, although these cells were not responsive to LPS stimulation. The inability of MoAb to C. neoformans capsular polysaccharide to promote IL-12 production by neutrophils excludes phagocytosis and/or CD16 cross-linking in this process, and distinguishes neutrophils from monocytes. Our results provide additional evidence for cytokine dysregulation in neutrophils from HIV-infected patients: Furthermore, the IL-12 response of neutrophils and monocytes to CD16 stimulation appears to be different, suggesting differences in the role of these phagocytic cells during the inflammatory response.

Original languageEnglish (US)
Pages (from-to)311-319
Number of pages9
JournalClinical and Experimental Immunology
Volume121
Issue number2
DOIs
StatePublished - 2000
Externally publishedYes

Fingerprint

Interleukin-12
Neutrophils
HIV
Cryptococcus neoformans
Lipopolysaccharides
Candida albicans
Monocytes
Tissue Donors
Cytokines
Phagocytes
Phagocytosis
Polysaccharides

Keywords

  • Antibodies
  • Cryptococcus neoformans
  • HIV
  • IL-12
  • Neutrophils

ASJC Scopus subject areas

  • Immunology

Cite this

Dysregulation in IL-12 secretion by neutrophils from HIV-infected patients. / Vecchiarelli, A.; Monari, C.; Palazzetti, B.; Bistoni, F.; Casadevall, Arturo.

In: Clinical and Experimental Immunology, Vol. 121, No. 2, 2000, p. 311-319.

Research output: Contribution to journalArticle

Vecchiarelli, A. ; Monari, C. ; Palazzetti, B. ; Bistoni, F. ; Casadevall, Arturo. / Dysregulation in IL-12 secretion by neutrophils from HIV-infected patients. In: Clinical and Experimental Immunology. 2000 ; Vol. 121, No. 2. pp. 311-319.
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