Dynamics of the bacterial intermediate filament crescentin in vitroand in vivo

Osigwe Esue, Laura Rupprecht, Sean X. Sun, Denis Wirtz

Research output: Contribution to journalArticle

Abstract

Background: Crescentin, the recently discovered bacterial intermediate filament protein, organizes into an extended filamentous structure that spans the length of the bacterium Caulobacter crescentus and plays a critical role in defining its curvature. The mechanism by which crescentin mediates cell curvature and whether crescentin filamentous structures are dynamic and/or polar are not fully understood. Methodology/Principal Findings:Using light microscopy, electron microscopy and quantitative rheology, we investigated the mechanics and dynamics of crescentin structures. Live-cell microscopy reveals that crescentin forms structures in vivo that undergo slow remodeling. The exchange of subunits between these structures and a pool of unassembled subunits is slow during the life cycle of the cell however; in vitro assembly and gelation of C. crescentus crescentin structures are rapid. Moreover, crescentin forms filamentous structures that are elastic, solid-like, and, like other intermediate filaments, can recover a significant portion of their network elasticity after shear. The assembly efficiency of crescentin is largely unaffected by monovalent cations (K+, Na+), but is enhanced by divalent cations (Mg2+, Ca2+), suggesting that the assembly kinetics and micromechanics of crescentin depend on the valence of the ions present in solution. Conclusions/Significance: These results indicate that crescentin forms filamentous structures that are elastic, labile, and stiff, and that their low dissociation rate from established structures controls the slow remodeling of crescentin in C. crescentus.

Original languageEnglish (US)
Article numbere8855
JournalPLoS One
Volume5
Issue number1
DOIs
StatePublished - Jan 25 2010

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Caulobacter crescentus
intermediate filaments
Intermediate Filaments
Microscopy
cations
intermediate filament proteins
Intermediate Filament Proteins
Monovalent Cations
bacterial proteins
Bacterial Proteins
Micromechanics
Rheology
Divalent Cations
Elasticity
rheology
cells
Gelation
gelation
elasticity (mechanics)
Life Cycle Stages

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Dynamics of the bacterial intermediate filament crescentin in vitroand in vivo. / Esue, Osigwe; Rupprecht, Laura; Sun, Sean X.; Wirtz, Denis.

In: PLoS One, Vol. 5, No. 1, e8855, 25.01.2010.

Research output: Contribution to journalArticle

Esue, Osigwe ; Rupprecht, Laura ; Sun, Sean X. ; Wirtz, Denis. / Dynamics of the bacterial intermediate filament crescentin in vitroand in vivo. In: PLoS One. 2010 ; Vol. 5, No. 1.
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