Dynamics in uracil base excision repair intrinsic dynamics drive the search for a damaged base

Joshua I. Friedman, James Stivers

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

The base excision repair enzyme human uracil DNA glycosylase (hUNG) exhibits dynamic fluctuations when bound to DNA that are consistent with transitioning between linear scanning and a pausing mode in which extrahelical thymine and uracil bases are interrogated. The absence of these motions in free UNG suggests that the enzyme uses the favorable free energy of DNA binding to loosen its own structure and activate dynamic modes necessary for the identification of damaged uracil bases.

Original languageEnglish (US)
Title of host publicationACS Symposium Series
PublisherAmerican Chemical Society
Pages47-58
Number of pages12
Volume1041
ISBN (Print)9780841225749
DOIs
StatePublished - Jun 1 2010

Publication series

NameACS Symposium Series
Volume1041
ISSN (Print)00976156
ISSN (Electronic)19475918

Fingerprint

Uracil
DNA
Repair
Uracil-DNA Glycosidase
Thymine
Enzymes
Free energy
Scanning

ASJC Scopus subject areas

  • Chemistry(all)
  • Chemical Engineering(all)

Cite this

Friedman, J. I., & Stivers, J. (2010). Dynamics in uracil base excision repair intrinsic dynamics drive the search for a damaged base. In ACS Symposium Series (Vol. 1041, pp. 47-58). (ACS Symposium Series; Vol. 1041). American Chemical Society. https://doi.org/10.1021/bk-2010-1041.ch004

Dynamics in uracil base excision repair intrinsic dynamics drive the search for a damaged base. / Friedman, Joshua I.; Stivers, James.

ACS Symposium Series. Vol. 1041 American Chemical Society, 2010. p. 47-58 (ACS Symposium Series; Vol. 1041).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Friedman, JI & Stivers, J 2010, Dynamics in uracil base excision repair intrinsic dynamics drive the search for a damaged base. in ACS Symposium Series. vol. 1041, ACS Symposium Series, vol. 1041, American Chemical Society, pp. 47-58. https://doi.org/10.1021/bk-2010-1041.ch004
Friedman JI, Stivers J. Dynamics in uracil base excision repair intrinsic dynamics drive the search for a damaged base. In ACS Symposium Series. Vol. 1041. American Chemical Society. 2010. p. 47-58. (ACS Symposium Series). https://doi.org/10.1021/bk-2010-1041.ch004
Friedman, Joshua I. ; Stivers, James. / Dynamics in uracil base excision repair intrinsic dynamics drive the search for a damaged base. ACS Symposium Series. Vol. 1041 American Chemical Society, 2010. pp. 47-58 (ACS Symposium Series).
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