Dynamic visualization of calcium-dependent signaling in cellular microdomains

Sohum Mehta, Jin Zhang

Research output: Contribution to journalArticle

Abstract

Cells rely on the coordinated action of diverse signaling molecules to sense, interpret, and respond to their highly dynamic external environment. To ensure the specific and robust flow of information, signaling molecules are often spatially organized to form distinct signaling compartments, and our understanding of the molecular mechanisms that guide intracellular signaling hinges on the ability to directly probe signaling events within these cellular microdomains. Ca2+ signaling in particular owes much of its functional versatility to this type of exquisite spatial regulation. As discussed below, a number of methods have been developed to investigate the mechanistic and functional implications of microdomains of Ca2+ signaling, ranging from the application of Ca2+ buffers to the direct and targeted visualization of Ca2+ signaling microdomains using genetically encoded fluorescent reporters.

Original languageEnglish (US)
Pages (from-to)333-341
Number of pages9
JournalCell Calcium
Volume58
Issue number4
DOIs
StatePublished - Oct 1 2015

Keywords

  • Biosensors
  • Calcineurin
  • Calmodulin
  • Compartmentalized signaling
  • FRET
  • Live-cell imaging

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology
  • Physiology

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