Dynamic usage of alternative splicing exons during mouse retina development

Jun Wan, Tomohiro Masuda, Laszlo Hackler, Kieron M. Torres, Shannath L Merbs, Donald J Zack, Jiang Qian

Research output: Contribution to journalArticle

Abstract

Alternative processing of pre-mRNA plays an important role in protein diversity and biological function. Previous studies on alternative splicing (AS) often focused on the spatial patterns of protein isoforms across different tissues. Here we studied dynamic usage of AS across time, during murine retina development. Over 7000 exons showed dynamical changes in splicing, with differential splicing events occurring more frequently in early development. The overall splicing patterns for exclusive and inclusive exons show symmetric trends and genes with symmetric splicing patterns that tend to have similar biological functions. Furthermore, we observed that within the retina, retina-enriched genes that are preferentially expressed at the adult stage tend to have more dynamically spliced exons compared to other genes, suggesting that genes maintaining retina homeostasis also play an important role in development via a series of AS events. Interestingly, the transcriptomes of retina-enriched genes largely reflect the retinal developmental process. Finally, we identified a number of candidate cis-regulatory elements for retinal AS by analyzing the relative occurrence of sequence motifs in exons or flanking introns. The occurrence of predicted regulatory elements showed strong correlation with the expression level of known RNA binding proteins, suggesting the high quality of the identified cis-regulatory elements.

Original languageEnglish (US)
Pages (from-to)7920-7930
Number of pages11
JournalNucleic Acids Research
Volume39
Issue number18
DOIs
StatePublished - Oct 2011

Fingerprint

Alternative Splicing
Retina
Exons
Genes
RNA-Binding Proteins
Biodiversity
RNA Precursors
Transcriptome
Introns
Protein Isoforms
Homeostasis
Proteins

ASJC Scopus subject areas

  • Genetics

Cite this

Dynamic usage of alternative splicing exons during mouse retina development. / Wan, Jun; Masuda, Tomohiro; Hackler, Laszlo; Torres, Kieron M.; Merbs, Shannath L; Zack, Donald J; Qian, Jiang.

In: Nucleic Acids Research, Vol. 39, No. 18, 10.2011, p. 7920-7930.

Research output: Contribution to journalArticle

Wan, Jun ; Masuda, Tomohiro ; Hackler, Laszlo ; Torres, Kieron M. ; Merbs, Shannath L ; Zack, Donald J ; Qian, Jiang. / Dynamic usage of alternative splicing exons during mouse retina development. In: Nucleic Acids Research. 2011 ; Vol. 39, No. 18. pp. 7920-7930.
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