Dynamic recruitment of phospholipase Cγ at transiently immobilized GPI-anchored receptor clusters induces IP₃-Ca²⁺ signaling: Single-molecule tracking study 2

Clusters of CD59, a glycosylphosphatidylinositol-anchored receptor (GPI-AR), with physiological sizes of approximately six CD59 molecules, recruit Gαi2 and Lyn via protein-protein and raft interactions. Lyn is activated probably by the Gαi2 binding in the same CD59 cluster, inducing the CD59 cluster's binding to F-actin, resulting in its immobilization, termed stimulation-induced temporary arrest of lateral diffusion (STALL; with a 0.57-s lifetime, occurring approximately every 2 s). Simultaneous single-molecule tracking of GFP-PLCγ2 and CD59 clusters revealed that PLCγ2 molecules are transiently (median = 0.25 s) recruited from the cytoplasm exclusively at the CD59 clusters undergoing STALL, producing the IP ₃-Ca²⁺ signal. Therefore, we propose that the CD59 cluster in STALL may be a key, albeit transient, platform for transducing the extracellular GPI-AR signal to the intracellular IP₃-Ca²⁺ signal, via PLCγ2 recruitment. The prolonged, analogue, bulk IP ₃-Ca²⁺ signal, which lasts for more than several minutes, is likely generated by the sum of the short-lived, digital-like IP₃ bursts, each created by the transient recruitment of PLCγ2 molecules to STALLed CD59.