Dynamic nuclear actin assembly by Arp2/3 complex and a baculovirus WASP-like protein

Erin D Goley, Taro Ohkawa, Joel Mancuso, Jeffrey B. Woodruff, Joseph A. D'Alessio, W. Zacheus Canda, Loy E. Volkman, Matthew D. Welch

Research output: Contribution to journalArticle

Abstract

Diverse bacterial and viral pathogens induce actin polymerization in the cytoplasm of host cells to facilitate infection. Here, we describe a pathogenic mechanism for promoting dynamic actin assembly in the nucleus to enable viral replication. The baculovirus Autographa californica multiple nucleopolyhedrovirus induced nuclear actin polymerization by translocating the host actin-nucleating Arp2/3 complex into the nucleus, where it was activated by p78/83, a viral Wiskott-Aldrich syndrome protein (WASP)-like protein. Nuclear actin assembly by p78/83 and Arp2/3 complex was essential for viral progeny production. Recompartmentalizing dynamic host actin may represent a conserved mode of pathogenesis and reflect viral manipulation of normal functions of nuclear actin.

Original languageEnglish (US)
Pages (from-to)464-467
Number of pages4
JournalScience
Volume314
Issue number5798
DOIs
StatePublished - Oct 20 2006
Externally publishedYes

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Wiskott-Aldrich Syndrome Protein
Actin-Related Protein 2-3 Complex
Baculoviridae
Actins
Proteins
Polymerization
Nucleopolyhedrovirus
Viral Proteins
Cytoplasm

ASJC Scopus subject areas

  • General

Cite this

Goley, E. D., Ohkawa, T., Mancuso, J., Woodruff, J. B., D'Alessio, J. A., Canda, W. Z., ... Welch, M. D. (2006). Dynamic nuclear actin assembly by Arp2/3 complex and a baculovirus WASP-like protein. Science, 314(5798), 464-467. https://doi.org/10.1126/science.1133348

Dynamic nuclear actin assembly by Arp2/3 complex and a baculovirus WASP-like protein. / Goley, Erin D; Ohkawa, Taro; Mancuso, Joel; Woodruff, Jeffrey B.; D'Alessio, Joseph A.; Canda, W. Zacheus; Volkman, Loy E.; Welch, Matthew D.

In: Science, Vol. 314, No. 5798, 20.10.2006, p. 464-467.

Research output: Contribution to journalArticle

Goley, ED, Ohkawa, T, Mancuso, J, Woodruff, JB, D'Alessio, JA, Canda, WZ, Volkman, LE & Welch, MD 2006, 'Dynamic nuclear actin assembly by Arp2/3 complex and a baculovirus WASP-like protein', Science, vol. 314, no. 5798, pp. 464-467. https://doi.org/10.1126/science.1133348
Goley ED, Ohkawa T, Mancuso J, Woodruff JB, D'Alessio JA, Canda WZ et al. Dynamic nuclear actin assembly by Arp2/3 complex and a baculovirus WASP-like protein. Science. 2006 Oct 20;314(5798):464-467. https://doi.org/10.1126/science.1133348
Goley, Erin D ; Ohkawa, Taro ; Mancuso, Joel ; Woodruff, Jeffrey B. ; D'Alessio, Joseph A. ; Canda, W. Zacheus ; Volkman, Loy E. ; Welch, Matthew D. / Dynamic nuclear actin assembly by Arp2/3 complex and a baculovirus WASP-like protein. In: Science. 2006 ; Vol. 314, No. 5798. pp. 464-467.
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