Dynamic imaging of allogeneic mesenchymal stem cells trafficking to myocardial infarction

Dara Kraitchman, Mitsuaki Tatsumi, Wesley D. Gilson, Takayoshi Ishimori, Dorota Kedziorek, Piotr Walczak, W. Paul Segars, Hunter H. Chen, Danielle Fritzges, Izlem Izbudak, Randell G. Young, Michelle Marcelino, Mark F. Pittenger, Meiyappan Solaiyappan, Raymond C. Boston, Benjamin Tsui, Richard L. Wahl, Jeff W Bulte

Research output: Contribution to journalArticle

Abstract

Background - Recent results from animal studies suggest that stem cells may be able to home to sites of myocardial injury to assist in tissue regeneration. However, the histological interpretation of postmortem tissue, on which many of these studies are based, has recently been widely debated. Methods and Results - With the use of the high sensitivity of a combined single-photon emission CT (SPECT)/CT scanner, the in vivo trafficking of allogeneic mesenchymal stem cells (MSCs) colabeled with a radiotracer and MR contrast agent to acute myocardial infarction was dynamically determined. Redistribution of the labeled MSCs after intravenous injection from initial localization in the lungs to nontarget organs such as the liver, kidney, and spleen was observed within 24 to 48 hours after injection. Focal and diffuse uptake of MSCs in the infarcted myocardium was already visible in SPECT/CT images in the first 24 hours after injection and persisted until 7 days after injection and was validated by tissue counts of radioactivity. In contrast, MRI was unable to demonstrate targeted cardiac localization of MSCs in part because of the lower sensitivity of MRI. Conclusions - Noninvasive radionuclide imaging is well suited to dynamically track the biodistribution and trafficking of mesenchymal stem cells to both target and nontarget organs.

Original languageEnglish (US)
Pages (from-to)1451-1461
Number of pages11
JournalCirculation
Volume112
Issue number10
DOIs
StatePublished - Sep 6 2005

Fingerprint

Mesenchymal Stromal Cells
Myocardial Infarction
Photons
Injections
Intravenous Injections
Radionuclide Imaging
Radioactivity
Contrast Media
Regeneration
Myocardium
Stem Cells
Spleen
Kidney
Lung
Liver
Wounds and Injuries

Keywords

  • Magnetic resonance imaging
  • Myocardial infarction
  • Nuclear medicine
  • Radionuclide imaging
  • Stem cell

ASJC Scopus subject areas

  • Physiology
  • Cardiology and Cardiovascular Medicine

Cite this

Dynamic imaging of allogeneic mesenchymal stem cells trafficking to myocardial infarction. / Kraitchman, Dara; Tatsumi, Mitsuaki; Gilson, Wesley D.; Ishimori, Takayoshi; Kedziorek, Dorota; Walczak, Piotr; Segars, W. Paul; Chen, Hunter H.; Fritzges, Danielle; Izbudak, Izlem; Young, Randell G.; Marcelino, Michelle; Pittenger, Mark F.; Solaiyappan, Meiyappan; Boston, Raymond C.; Tsui, Benjamin; Wahl, Richard L.; Bulte, Jeff W.

In: Circulation, Vol. 112, No. 10, 06.09.2005, p. 1451-1461.

Research output: Contribution to journalArticle

Kraitchman, D, Tatsumi, M, Gilson, WD, Ishimori, T, Kedziorek, D, Walczak, P, Segars, WP, Chen, HH, Fritzges, D, Izbudak, I, Young, RG, Marcelino, M, Pittenger, MF, Solaiyappan, M, Boston, RC, Tsui, B, Wahl, RL & Bulte, JW 2005, 'Dynamic imaging of allogeneic mesenchymal stem cells trafficking to myocardial infarction', Circulation, vol. 112, no. 10, pp. 1451-1461. https://doi.org/10.1161/CIRCULATIONAHA.105.537480
Kraitchman, Dara ; Tatsumi, Mitsuaki ; Gilson, Wesley D. ; Ishimori, Takayoshi ; Kedziorek, Dorota ; Walczak, Piotr ; Segars, W. Paul ; Chen, Hunter H. ; Fritzges, Danielle ; Izbudak, Izlem ; Young, Randell G. ; Marcelino, Michelle ; Pittenger, Mark F. ; Solaiyappan, Meiyappan ; Boston, Raymond C. ; Tsui, Benjamin ; Wahl, Richard L. ; Bulte, Jeff W. / Dynamic imaging of allogeneic mesenchymal stem cells trafficking to myocardial infarction. In: Circulation. 2005 ; Vol. 112, No. 10. pp. 1451-1461.
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AU - Tatsumi, Mitsuaki

AU - Gilson, Wesley D.

AU - Ishimori, Takayoshi

AU - Kedziorek, Dorota

AU - Walczak, Piotr

AU - Segars, W. Paul

AU - Chen, Hunter H.

AU - Fritzges, Danielle

AU - Izbudak, Izlem

AU - Young, Randell G.

AU - Marcelino, Michelle

AU - Pittenger, Mark F.

AU - Solaiyappan, Meiyappan

AU - Boston, Raymond C.

AU - Tsui, Benjamin

AU - Wahl, Richard L.

AU - Bulte, Jeff W

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N2 - Background - Recent results from animal studies suggest that stem cells may be able to home to sites of myocardial injury to assist in tissue regeneration. However, the histological interpretation of postmortem tissue, on which many of these studies are based, has recently been widely debated. Methods and Results - With the use of the high sensitivity of a combined single-photon emission CT (SPECT)/CT scanner, the in vivo trafficking of allogeneic mesenchymal stem cells (MSCs) colabeled with a radiotracer and MR contrast agent to acute myocardial infarction was dynamically determined. Redistribution of the labeled MSCs after intravenous injection from initial localization in the lungs to nontarget organs such as the liver, kidney, and spleen was observed within 24 to 48 hours after injection. Focal and diffuse uptake of MSCs in the infarcted myocardium was already visible in SPECT/CT images in the first 24 hours after injection and persisted until 7 days after injection and was validated by tissue counts of radioactivity. In contrast, MRI was unable to demonstrate targeted cardiac localization of MSCs in part because of the lower sensitivity of MRI. Conclusions - Noninvasive radionuclide imaging is well suited to dynamically track the biodistribution and trafficking of mesenchymal stem cells to both target and nontarget organs.

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