Dye-mediated photolysis of normal and neoplastic hematopoietic cells

Fritz Sieber, Robert K. Stuart, Scott D. Rowley, Saul J. Sharkis, Lyle L. Sensenbrenner

Research output: Contribution to journalArticle

Abstract

The purpose of this study was to determine the sensitivity to merocyanine 540 (MC 540)-mediated photolysis of normal human hematopoietic progenitor cells and four leukemia cell lines (Daudi, Raji, K562 and HL-60). Late erythroid progenitors were the most sensitive normal cells. Early erythroid progenitors were of intermediate sensitivity. Granulocyte/macrophage progenitors and multipotent progenitors were the least sensitive normal marrow cells. A combination of dye concentration, serum concentration, and illumination that eliminated 50% of multipotent progenitor cells reduced the concentration of leukemic cells by ≥4.5 log. It is conceivable that this difference in photosensitivity can be exploited for the extracorporeal purging of autologous remission marrow grafts.

Original languageEnglish (US)
Pages (from-to)43-49
Number of pages7
JournalLeukemia Research
Volume11
Issue number1
DOIs
StatePublished - 1987

Keywords

  • Autologous marrow transplantation
  • extracorporeal purging
  • hematopoietic stem cells
  • leukemia cells
  • merocyanine 540
  • photosensitization

ASJC Scopus subject areas

  • Hematology
  • Oncology
  • Cancer Research

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  • Cite this

    Sieber, F., Stuart, R. K., Rowley, S. D., Sharkis, S. J., & Sensenbrenner, L. L. (1987). Dye-mediated photolysis of normal and neoplastic hematopoietic cells. Leukemia Research, 11(1), 43-49. https://doi.org/10.1016/0145-2126(87)90104-4