Dye-mediated photolysis of normal and neoplastic hematopoietic cells

Fritz Sieber; Robert K. Stuart; Scott D. Rowley; Saul J. Sharkis; Lyle L. Sensenbrenner

doi:10.1016/0145-2126(87)90104-4

Dye-mediated photolysis of normal and neoplastic hematopoietic cells

Fritz Sieber, Robert K. Stuart, Scott D. Rowley, Saul J. Sharkis, Lyle L. Sensenbrenner

School of Medicine

Research output: Contribution to journal › Article › peer-review

57 Scopus citations

Abstract

The purpose of this study was to determine the sensitivity to merocyanine 540 (MC 540)-mediated photolysis of normal human hematopoietic progenitor cells and four leukemia cell lines (Daudi, Raji, K562 and HL-60). Late erythroid progenitors were the most sensitive normal cells. Early erythroid progenitors were of intermediate sensitivity. Granulocyte/macrophage progenitors and multipotent progenitors were the least sensitive normal marrow cells. A combination of dye concentration, serum concentration, and illumination that eliminated 50% of multipotent progenitor cells reduced the concentration of leukemic cells by ≥4.5 log. It is conceivable that this difference in photosensitivity can be exploited for the extracorporeal purging of autologous remission marrow grafts.

Original language	English (US)
Pages (from-to)	43-49
Number of pages	7
Journal	Leukemia Research
Volume	11
Issue number	1
DOIs	https://doi.org/10.1016/0145-2126(87)90104-4
State	Published - 1987

Keywords

Autologous marrow transplantation
extracorporeal purging
hematopoietic stem cells
leukemia cells
merocyanine 540
photosensitization

ASJC Scopus subject areas

Hematology
Oncology
Cancer Research

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10.1016/0145-2126(87)90104-4

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title = "Dye-mediated photolysis of normal and neoplastic hematopoietic cells",

abstract = "The purpose of this study was to determine the sensitivity to merocyanine 540 (MC 540)-mediated photolysis of normal human hematopoietic progenitor cells and four leukemia cell lines (Daudi, Raji, K562 and HL-60). Late erythroid progenitors were the most sensitive normal cells. Early erythroid progenitors were of intermediate sensitivity. Granulocyte/macrophage progenitors and multipotent progenitors were the least sensitive normal marrow cells. A combination of dye concentration, serum concentration, and illumination that eliminated 50% of multipotent progenitor cells reduced the concentration of leukemic cells by ≥4.5 log. It is conceivable that this difference in photosensitivity can be exploited for the extracorporeal purging of autologous remission marrow grafts.",

keywords = "Autologous marrow transplantation, extracorporeal purging, hematopoietic stem cells, leukemia cells, merocyanine 540, photosensitization",

author = "Fritz Sieber and Stuart, {Robert K.} and Rowley, {Scott D.} and Sharkis, {Saul J.} and Sensenbrenner, {Lyle L.}",

note = "Funding Information: Acknowledgements--We thank Dr G. W. Santos for stimulating discussions. Dr R. C. Ash for clinical specimens. G. J. Krueger. M. W. Corcoran. M. Isaacs. E. Connor. and M. Zicha for excellent technical assistance, and C. Snvder for the illustrations. This work was supported by grant AM 27157 from the National Institute of Arthritis, Diabetes and Digestive and Kidney Diseases. grant CA 42734 from the National Cancer Institute and an equipment grant from the MACC Fund. F.S. and S.J.S. are Leukemia Society of America Scholars and R.K.S. is a Leukemia Society of Amcrica Special Fellow. Copyright: Copyright 2015 Elsevier B.V., All rights reserved.",

year = "1987",

doi = "10.1016/0145-2126(87)90104-4",

language = "English (US)",

volume = "11",

pages = "43--49",

journal = "Leukemia Research",

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AU - Sieber, Fritz

AU - Stuart, Robert K.

AU - Rowley, Scott D.

AU - Sharkis, Saul J.

AU - Sensenbrenner, Lyle L.

N1 - Funding Information: Acknowledgements--We thank Dr G. W. Santos for stimulating discussions. Dr R. C. Ash for clinical specimens. G. J. Krueger. M. W. Corcoran. M. Isaacs. E. Connor. and M. Zicha for excellent technical assistance, and C. Snvder for the illustrations. This work was supported by grant AM 27157 from the National Institute of Arthritis, Diabetes and Digestive and Kidney Diseases. grant CA 42734 from the National Cancer Institute and an equipment grant from the MACC Fund. F.S. and S.J.S. are Leukemia Society of America Scholars and R.K.S. is a Leukemia Society of Amcrica Special Fellow. Copyright: Copyright 2015 Elsevier B.V., All rights reserved.

PY - 1987

Y1 - 1987

N2 - The purpose of this study was to determine the sensitivity to merocyanine 540 (MC 540)-mediated photolysis of normal human hematopoietic progenitor cells and four leukemia cell lines (Daudi, Raji, K562 and HL-60). Late erythroid progenitors were the most sensitive normal cells. Early erythroid progenitors were of intermediate sensitivity. Granulocyte/macrophage progenitors and multipotent progenitors were the least sensitive normal marrow cells. A combination of dye concentration, serum concentration, and illumination that eliminated 50% of multipotent progenitor cells reduced the concentration of leukemic cells by ≥4.5 log. It is conceivable that this difference in photosensitivity can be exploited for the extracorporeal purging of autologous remission marrow grafts.

AB - The purpose of this study was to determine the sensitivity to merocyanine 540 (MC 540)-mediated photolysis of normal human hematopoietic progenitor cells and four leukemia cell lines (Daudi, Raji, K562 and HL-60). Late erythroid progenitors were the most sensitive normal cells. Early erythroid progenitors were of intermediate sensitivity. Granulocyte/macrophage progenitors and multipotent progenitors were the least sensitive normal marrow cells. A combination of dye concentration, serum concentration, and illumination that eliminated 50% of multipotent progenitor cells reduced the concentration of leukemic cells by ≥4.5 log. It is conceivable that this difference in photosensitivity can be exploited for the extracorporeal purging of autologous remission marrow grafts.

KW - Autologous marrow transplantation

KW - extracorporeal purging

KW - hematopoietic stem cells

KW - leukemia cells

KW - merocyanine 540

KW - photosensitization

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Dye-mediated photolysis of normal and neoplastic hematopoietic cells

Abstract

Keywords

ASJC Scopus subject areas

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