Dual-SMAD Inhibition/WNT Activation-Based Methods to Induce Neural Crest and Derivatives from Human Pluripotent Stem Cells

Stuart M. Chambers, Yvonne Mica, Gabsang Lee, Lorenz Studer, Mark J. Tomishima

Research output: Contribution to journalArticlepeer-review

Abstract

The neural crest (NC) is a transient population of multipotent cells giving rise to the peripheral nervous system, skin pigmentation, heart, and facial mesenchyme. The broad cell fate potential of NC makes it an attractive cell fate to derive from human pluripotent stem cells (hPSCs) for exploring embryonic deve lopment,modeling disease, and generating cells for transplantation. Here, we discuss recent publications and methods for efficiently differentiating hPSCs into NC. We also provide methods to direct NC into two different terminal fates: melanocytes and sensory neurons.

Original languageEnglish (US)
Pages (from-to)329-343
Number of pages15
JournalMethods in Molecular Biology
Volume1307
DOIs
StatePublished - 2015

Keywords

  • Disease modeling
  • Dual SMAD inhibition
  • Human pluripotent stem cells
  • Melanocyte
  • Neural crest
  • Peripheral sensory neurons

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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