TY - JOUR
T1 - Dual-Reporter Drift Correction To Enhance the Performance of Electrochemical Aptamer-Based Sensors in Whole Blood
AU - Li, Hui
AU - Arroyo-Currás, Netzahualcóyotl
AU - Kang, Di
AU - Ricci, Francesco
AU - Plaxco, Kevin W.
N1 - Funding Information:
The biosensor work at UCSB was carried out at the Institute for Collaborative Biotechnologies (supported by the Army Research Office, Grant W911NF-09-0001) and with partial support from the W. M. Keck Foundation. Dr. Li was partially supported by the Swiss National Science Foundation with an “Early Postdoc Mobility fellowship”. Dr. Li also appreciates helpful discussions with Dr. Shaoguang Li, Dr. Philippe Dauphin Ducharme, and Dr. Martin Kurnik.
Publisher Copyright:
© 2016 American Chemical Society
PY - 2016/12/14
Y1 - 2016/12/14
N2 - The continuous, real-time monitoring of specific molecular targets in unprocessed clinical samples would enable many transformative medical applications. Electrochemical aptamer-based (E-AB) sensors appear to be a promising approach to this end because of their selectivity (performance in complex samples, such as serum) and reversible, single-step operation. E-AB sensors suffer, however, from often-severe baseline drift when challenged in undiluted whole blood. In response we report here a dual-reporter approach to performing E-AB baseline drift correction. The approach incorporates two redox reporters on the aptamer, one of which serves as the target-responsive sensor and the other, which reports at a distinct, nonoverlapping redox potential, serving as a drift-correcting reference. Taking the difference in their relative signals largely eliminates the drift observed for these sensors in flowing, undiluted whole blood, reducing drift of up to 50% to less than 2% over many hours of continuous operation under these challenging conditions.
AB - The continuous, real-time monitoring of specific molecular targets in unprocessed clinical samples would enable many transformative medical applications. Electrochemical aptamer-based (E-AB) sensors appear to be a promising approach to this end because of their selectivity (performance in complex samples, such as serum) and reversible, single-step operation. E-AB sensors suffer, however, from often-severe baseline drift when challenged in undiluted whole blood. In response we report here a dual-reporter approach to performing E-AB baseline drift correction. The approach incorporates two redox reporters on the aptamer, one of which serves as the target-responsive sensor and the other, which reports at a distinct, nonoverlapping redox potential, serving as a drift-correcting reference. Taking the difference in their relative signals largely eliminates the drift observed for these sensors in flowing, undiluted whole blood, reducing drift of up to 50% to less than 2% over many hours of continuous operation under these challenging conditions.
UR - http://www.scopus.com/inward/record.url?scp=85006241190&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85006241190&partnerID=8YFLogxK
U2 - 10.1021/jacs.6b08671
DO - 10.1021/jacs.6b08671
M3 - Article
C2 - 27960346
AN - SCOPUS:85006241190
SN - 0002-7863
VL - 138
SP - 15809
EP - 15812
JO - Journal of the American Chemical Society
JF - Journal of the American Chemical Society
IS - 49
ER -