Dual-modality fiber-optic imager (DFOI) for intracellular gene delivery in human cervical cancer cell

Jaepyeong Cha, Jing Zhang, Saumya Gurbani, Min Li, Jin U. Kang

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

The most common optical method to validate intracellular gene delivery in cancer is to detect tagged fluorescence signals from the cells. However, fluorescent detection is usually performed in vitro due to the limitation of standard microscopes. Herein, we propose a highly sensitive dual-modality fiber-optic imager (DFOI), which enables in vivo fluorescence imaging. Our system uses a coherent fiber bundle based imager capable of simultaneously performing both confocal reflectance and fluorescent microscopy. Non-viral vectors targeting human cervical cancer cells (HeLa) were used to evaluate the performance. Preliminary results demonstrated the DFOI is promising for in vivo evaluation of intracellular gene delivery.

Original languageEnglish (US)
Title of host publicationOptical Fibers and Sensors for Medical Diagnostics and Treatment Applications XIII
DOIs
StatePublished - May 22 2013
EventOptical Fibers and Sensors for Medical Diagnostics and Treatment Applications XIII - San Francisco, CA, United States
Duration: Feb 2 2013Feb 3 2013

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume8576
ISSN (Print)1605-7422

Other

OtherOptical Fibers and Sensors for Medical Diagnostics and Treatment Applications XIII
CountryUnited States
CitySan Francisco, CA
Period2/2/132/3/13

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Keywords

  • fiber-optic imaging
  • fluorescent endomicroscopy
  • intracellular gene delivery

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Atomic and Molecular Physics, and Optics
  • Radiology Nuclear Medicine and imaging

Cite this

Cha, J., Zhang, J., Gurbani, S., Li, M., & Kang, J. U. (2013). Dual-modality fiber-optic imager (DFOI) for intracellular gene delivery in human cervical cancer cell. In Optical Fibers and Sensors for Medical Diagnostics and Treatment Applications XIII [857604] (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 8576). https://doi.org/10.1117/12.2001636