TY - JOUR
T1 - Dual infection with human immunodeficiency virus type 1 of distinct envelope subtypes in humans
AU - Artenstein, Andrew W.
AU - Van Cott, Thomas C.
AU - Mascola, John R.
AU - Carr, Jean K.
AU - Hegerich, Patricia A.
AU - Gaywee, Jariyanart
AU - Sanders-Buell, Eric
AU - Robb, Merlin L.
AU - Dayhoff, Debora E.
AU - Thitivichianlert, Sataporn
AU - Nitayaphan, Sorachai
AU - Mc Neil, John G.
AU - Birx, Deborah L.
AU - Michael, Rodney A.
AU - Burke, Donald S.
AU - Mc Cutchan, Francine E.
N1 - Funding Information:
Received 31 August 1994; revised 22 November 1994. Presented in part: 34th Interscience Conference on Antimicrobial Agents and Chemotherapy. Orlando. Florida. 3-7 October 1994. Informed consent was obtained from human subjects. Financial support: Department of Defense. The views of the authors do not purport to reflect the opinions of the Departments of Army. Navy. or Defense. Sequences have been submitted to GenBank under accession nos. U21471-U21476. Reprints or correspondence: Dr. Andrew W. Artenstein, Division ofRetrovirology, Walter Reed Army Institute of Research. 1600 E. Gude Dr., Rockville. MD 20850.
PY - 1995/4
Y1 - 1995/4
N2 - Multiple genetic subtypes of human immunodeficiency virus type 1 (HIV-1) have been identified among internationally collected isolates. The HIV-1 epidemic in Thailand is largely due to B and E subtypes of virus. Dual infection with distinct HIV-1 subtypes would suggest that antiviral immunity evoked by one subtype can be incompletely protective against a second. Polymerase chain reaction typing and serologic typing were used to screen a panel of specimens from HIV-1infected subjects in Thailand. Two persons simultaneously harbored HIV-1 of env subtypes B and E, and this was confirmed by colony hybridization with subtype-specific probes and nucleotide sequence analysis of a 630-bp fragment of gp120 from multiple molecular clones. In addition, both subtypes were identified in cocultured peripheral blood mononuclear cells from 1 individual. These data provide the first evidence of dual HIV-1 infection in humans and reinforce the need for polyvalent vaccines.
AB - Multiple genetic subtypes of human immunodeficiency virus type 1 (HIV-1) have been identified among internationally collected isolates. The HIV-1 epidemic in Thailand is largely due to B and E subtypes of virus. Dual infection with distinct HIV-1 subtypes would suggest that antiviral immunity evoked by one subtype can be incompletely protective against a second. Polymerase chain reaction typing and serologic typing were used to screen a panel of specimens from HIV-1infected subjects in Thailand. Two persons simultaneously harbored HIV-1 of env subtypes B and E, and this was confirmed by colony hybridization with subtype-specific probes and nucleotide sequence analysis of a 630-bp fragment of gp120 from multiple molecular clones. In addition, both subtypes were identified in cocultured peripheral blood mononuclear cells from 1 individual. These data provide the first evidence of dual HIV-1 infection in humans and reinforce the need for polyvalent vaccines.
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U2 - 10.1093/infdis/171.4.805
DO - 10.1093/infdis/171.4.805
M3 - Article
C2 - 7706806
AN - SCOPUS:0028947028
VL - 171
SP - 805
EP - 810
JO - Journal of Infectious Diseases
JF - Journal of Infectious Diseases
SN - 0022-1899
IS - 4
ER -