Dual effects of bilirubin on the proliferation of rat renal NRK52E cells and its association with gap junctions

Yanling Wang, Qiongfang Zhu, Chenfang Luo, Ailan Zhang, Ziqing Hei, Guangjie Su, Zhengyuan Xia, Michael G. Irwin

Research output: Contribution to journalArticle

Abstract

Objective: The effect of bilirubin on renal pathophysiology is controversial. This study aimed to observe the effects of bilirubin on the proliferation of normal rat renal tubular epithelial cell line (NRK52E) and its potential interplay with gap junction function. Methods: Cultured NRK52E cells, seeded respectively at high- or low- densities, were treated with varying concentrations of bilirubin for 24 hours. Cell injury was assessed by measuring cell viability and proliferation, and gap junction function was assessed by Parachute dye-coupling assay. Connexin 43 protein was assessed by Western blotting. Results: At doses from 17.1 to 513μmol/L, bilirubin dose-dependently enhanced cell viability and colony-formation rates when cells were seeded at either high- or low- densities (all p

Original languageEnglish (US)
Pages (from-to)220-237
Number of pages18
JournalDose-Response
Volume11
Issue number2
DOIs
StatePublished - 2013
Externally publishedYes

Fingerprint

Gap Junctions
Bilirubin
Rats
Cells
Association reactions
Kidney
Parachutes
Assays
Cell Survival
Dyes
Proteins
Connexin 43
Cultured Cells
Coloring Agents
Western Blotting
Epithelial Cells
Cell Proliferation
Cell Line
Wounds and Injuries

Keywords

  • Bilirubin
  • Cell proliferation
  • Connexin
  • Gap junction
  • Kidney injury
  • NRK52E cells

ASJC Scopus subject areas

  • Toxicology
  • Health, Toxicology and Mutagenesis
  • Chemical Health and Safety
  • Public Health, Environmental and Occupational Health

Cite this

Dual effects of bilirubin on the proliferation of rat renal NRK52E cells and its association with gap junctions. / Wang, Yanling; Zhu, Qiongfang; Luo, Chenfang; Zhang, Ailan; Hei, Ziqing; Su, Guangjie; Xia, Zhengyuan; Irwin, Michael G.

In: Dose-Response, Vol. 11, No. 2, 2013, p. 220-237.

Research output: Contribution to journalArticle

Wang, Yanling ; Zhu, Qiongfang ; Luo, Chenfang ; Zhang, Ailan ; Hei, Ziqing ; Su, Guangjie ; Xia, Zhengyuan ; Irwin, Michael G. / Dual effects of bilirubin on the proliferation of rat renal NRK52E cells and its association with gap junctions. In: Dose-Response. 2013 ; Vol. 11, No. 2. pp. 220-237.
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AU - Hei, Ziqing

AU - Su, Guangjie

AU - Xia, Zhengyuan

AU - Irwin, Michael G.

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