Dual-color three-dimensional STED microscopy with a single high-repetition-rate laser

Kyu Young Han, Taekjip Ha

Research output: Contribution to journalArticlepeer-review

Abstract

We describe a dual-color three-dimensional stimulated emission depletion (3D-STED) microscopy employing a single laser source with a repetition rate of 80 MHz. Multiple excitation pulses synchronized with a STED pulse were generated by a photonic crystal fiber, and the desired wavelengths were selected by an acousto-optic tunable filter with high spectral purity. Selective excitation at different wavelengths permits simultaneous imaging of two fluorescent markers at a nanoscale resolution in three dimensions.

Original languageEnglish (US)
Pages (from-to)2653-2656
Number of pages4
JournalOptics Letters
Volume40
Issue number11
DOIs
StatePublished - Jun 1 2015
Externally publishedYes

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics

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