TY - JOUR
T1 - Drug absorption by the respiratory mucosa
T2 - Cell culture models and particulate drug carriers
AU - Ehrhardt, C.
AU - Fiegel, J.
AU - Fuchs, S.
AU - Abu-Dahab, R.
AU - Schaefer, U. F.
AU - Hanes, J.
AU - Lehr, Claus M.
PY - 2002
Y1 - 2002
N2 - The inhalation route is of increasing interest for both local and systemic drug delivery, including macromolecular biopharmaceuticals, such as peptides, proteins, and gene therapeutics. In addition to appropriate aerosolization for deposition in relevant areas of the respiratory tract, therapeutic molecules may require an advanced carrier system for safe and efficient delivery to their target. Two approaches to obtain novel carrier systems for pulmonary drug delivery are large porous microparticles with a low aerodynamic diameter and lectin-functionalized liposomes. Epithelial cells of alveolar or bronchial origin, obtained either from patient material or from established cell lines, can be grown on permeable filter supports, resulting in polarized monolayers with functional intercellular junctions. With such in vitro models, transport of drugs into pulmonary epithelial cells and/or across the air-blood barrier, as well as the effect and efficacy of novel drug carrier systems can be systematically studied.
AB - The inhalation route is of increasing interest for both local and systemic drug delivery, including macromolecular biopharmaceuticals, such as peptides, proteins, and gene therapeutics. In addition to appropriate aerosolization for deposition in relevant areas of the respiratory tract, therapeutic molecules may require an advanced carrier system for safe and efficient delivery to their target. Two approaches to obtain novel carrier systems for pulmonary drug delivery are large porous microparticles with a low aerodynamic diameter and lectin-functionalized liposomes. Epithelial cells of alveolar or bronchial origin, obtained either from patient material or from established cell lines, can be grown on permeable filter supports, resulting in polarized monolayers with functional intercellular junctions. With such in vitro models, transport of drugs into pulmonary epithelial cells and/or across the air-blood barrier, as well as the effect and efficacy of novel drug carrier systems can be systematically studied.
KW - Air-interface culture
KW - Alveolar epithelial cells
KW - Bronchial epithelial cells
KW - Lectins
KW - Liposomes
KW - Microparticles
UR - http://www.scopus.com/inward/record.url?scp=0036321111&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0036321111&partnerID=8YFLogxK
U2 - 10.1089/089426802320282257
DO - 10.1089/089426802320282257
M3 - Article
C2 - 12184863
AN - SCOPUS:0036321111
SN - 0894-2684
VL - 15
SP - 131
EP - 139
JO - Journal of Aerosol Medicine: Deposition, Clearance, and Effects in the Lung
JF - Journal of Aerosol Medicine: Deposition, Clearance, and Effects in the Lung
IS - 2
ER -