Drosophila TRPA1 channel mediates chemical avoidance in gustatory receptor neurons

Sang Hoon Kim, Youngseok Lee, Bradley Akitake, Owen M. Woodward, William B Guggino, Craig Montella

Research output: Contribution to journalArticle

Abstract

Mammalian sweet, bitter, and umami taste is mediated by a single transduction pathway that includes a phospholipase C (PLC)β and one cation channel, TRPM5. However, in insects such as the fruit fly, Drosophila melanogaster, it is unclear whether different tastants, such as bitter compounds, are sensed in gustatory receptor neurons (GRNs) through one or multiple ion channels, as the cation channels required in insect GRNs are unknown. Here, we set out to explore additional sensory roles for the Drosophila TRPA1 channel, which was known to function in thermosensation. We found that TRPA1 was expressed in GRNs that respond to aversive compounds. Elimination of TRPA1 had no impact on the responses to nearly all bitter compounds tested, including caffeine, quinine, and strychnine. Rather, we found that TRPA1 was required in a subset of avoidance GRNs for the behavioral and electrophysiological responses to aristolochic acid. TRPA1 did not appear to be activated or inhibited directly by aristolochic acid. We found that elimination of the same PLC that leads to activation of TRPA1 in thermosensory neurons was also required in the TRPA1-expressing GRNs for avoiding aristolochic acid. Given that mammalian TRPA1 is required for responding to noxious chemicals, many of which cause pain and injury, our analysis underscores the evolutionarily conserved role for TRPA1 channels in chemical avoidance.

Original languageEnglish (US)
Pages (from-to)8440-8445
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume107
Issue number18
DOIs
StatePublished - May 4 2010

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Drosophila
Neurons
Type C Phospholipases
Insects
Cations
Strychnine
Quinine
Caffeine
Drosophila melanogaster
Ion Channels
Diptera
Fruit
Pain
Wounds and Injuries
aristolochic acid I

Keywords

  • Aristolochic acid
  • Chemosensation
  • Phospholipase C
  • Taste
  • Transient receptor potential

ASJC Scopus subject areas

  • General

Cite this

Drosophila TRPA1 channel mediates chemical avoidance in gustatory receptor neurons. / Kim, Sang Hoon; Lee, Youngseok; Akitake, Bradley; Woodward, Owen M.; Guggino, William B; Montella, Craig.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 107, No. 18, 04.05.2010, p. 8440-8445.

Research output: Contribution to journalArticle

Kim, Sang Hoon ; Lee, Youngseok ; Akitake, Bradley ; Woodward, Owen M. ; Guggino, William B ; Montella, Craig. / Drosophila TRPA1 channel mediates chemical avoidance in gustatory receptor neurons. In: Proceedings of the National Academy of Sciences of the United States of America. 2010 ; Vol. 107, No. 18. pp. 8440-8445.
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