Drosophila Pxt: A cyclooxygenase-like facilitator of follicle maturation

Tina L. Tootle, Allan C. Spradling

Research output: Contribution to journalArticlepeer-review

85 Scopus citations


Prostaglandins are local transient hormones that mediate a wide variety of biological events, including reproduction. The study of prostaglandin biology in a genetically tractable invertebrate model organism has been limited by the lack of clearly identified prostaglandin-mediated biological processes and prostaglandin metabolic genes, particularly analogs of cyclooxygenase (COX), the rate-limiting step in vertebrate prostaglandin synthesis. Here, we present pharmacological data that Drosophila ovarian follicle maturation requires COX-like activity and genetic evidence that this activity is supplied in vivo by the Drosophila peroxidase Pxt. pxt mutant females are sterile, and maturing follicles show defects in actin filament formation, nurse cell membrane stability and border cell migration. Maturation of pxt follicles in vitro is stimulated by prostaglandin treatment and fertility is restored in vivo to pxt mutants by expressing mammalian Cox1 protein. Our experiments suggest that prostaglandins promote Drosophila follicle maturation, in part by modulating the actin cytoskeleton, and establish Drosophila oogenesis as a model for understanding these critical biological regulators.

Original languageEnglish (US)
Pages (from-to)839-847
Number of pages9
Issue number5
StatePublished - Mar 2008
Externally publishedYes


  • Actin
  • Cyclooxygenase
  • Oogenesis
  • Ovarian follicle
  • Prostaglandin

ASJC Scopus subject areas

  • Anatomy
  • Cell Biology


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